Snow A D, Mar H, Nochlin D, Kimata K, Kato M, Suzuki S, Hassell J, Wight T N
Department of Pathology, University of Washington, Seattle 98195.
Am J Pathol. 1988 Dec;133(3):456-63.
Two immunocytochemical probes were used to specifically identify and localize heparan sulphate proteoglycans (HSPGs) in 17 cases of Alzheimer's disease (AD). A monoclonal (HK-102) and an affinity-purified polyclonal antibody, each recognizing specific domains on the protein core of a basement membrane-derived HSPG, localized HSPGs to the amyloid fibrils present in neuritic plaques (NPs) and congophilic angiopathy (CA) in the brains of Alzheimer's patients, with weak to no immunostaining in neurofibrillary tangles from the same tissues. HSPGs were also demonstrated in "primitive plaques," suggesting that their accumulation takes place during early stages of plaque development. Immunolocalization of HSPGs to subsets of astrocytes and neuronal cells, particularly those in close proximity to NPs and CA, suggested possible involvement of these two cell types in deposition of HS-PGs into the amyloidotic lesions. The current study not only identifies a new component (HSPGs) present in the amyloid deposits of NPs and CA but also suggests that astrocytes, neurons, or both may be involved in its deposition at these sites.
使用两种免疫细胞化学探针，特异性鉴定并定位17例阿尔茨海默病（AD）患者脑中的硫酸乙酰肝素蛋白聚糖（HSPG）。一种单克隆抗体（HK-102）和一种亲和纯化的多克隆抗体，各自识别源自基底膜的HSPG蛋白核心上的特定结构域，将HSPG定位到阿尔茨海默病患者脑中神经炎性斑块（NP）和嗜刚果红血管病（CA）中存在的淀粉样纤维上，而相同组织的神经原纤维缠结中免疫染色较弱或无染色。在“原始斑块”中也证实了HSPG的存在，这表明它们在斑块形成的早期阶段就开始积累。HSPG在星形胶质细胞和神经元细胞亚群中的免疫定位，特别是那些紧邻NP和CA的细胞，提示这两种细胞类型可能参与了HS-PG在淀粉样病变中的沉积。当前研究不仅鉴定出NP和CA淀粉样沉积物中存在的一种新成分（HSPG），还表明星形胶质细胞、神经元或两者可能参与了其在这些部位的沉积。
