冻干金黄色葡萄球菌分型噬菌体的稳定性
Stabilities of lyophilized Staphylococcus aureus typing bacteriophages.
作者信息
Zierdt C H
机构信息
Department of Clinical Pathology, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
Appl Environ Microbiol. 1988 Oct;54(10):2590. doi: 10.1128/aem.54.10.2590-.1988.
Abstract
Staphylococcus aureus bacteriophages (25 phages) were lyophilized in aliquots 12 to 18 years ago and stored in vacuo at -20 degrees C. Eight viruses each lost one log titer, while seventeen retained the original titers. The use of lyophilized phages provided more reproducible phage typing and reduced by 75% the complexity and cost. This important test is thus made feasible for more laboratories.
摘要
金黄色葡萄球菌噬菌体（25种噬菌体）于12至18年前被冻干成分份保存，并在-20℃真空储存。其中8种病毒每种损失了一个对数滴度，而17种保留了原始滴度。使用冻干噬菌体可使噬菌体分型更具可重复性，并将复杂性和成本降低75%。因此，这项重要检测对更多实验室来说变得可行。
相似文献
1
Stabilities of lyophilized Staphylococcus aureus typing bacteriophages.
Appl Environ Microbiol. 1988 Oct;54(10):2590. doi: 10.1128/aem.54.10.2590-.1988.
2
A simplified approach to the phage typing of Staphylococcus aureus. I. The use of lyophilized phage.
Tech Bull Regist Med Technol. 1960 Jun;30:93-107.
3
Phage typing of nontypable isolates of Staphylococcus aureus using the new phages 94, 95, and 96 and the heat-shock treatment.
Am J Clin Pathol. 1978 Oct;70(4):686-8. doi: 10.1093/ajcp/70.4.686.
4
[Bacteriophage typing of Staphylococcus aureus in Turkey].
Mikrobiyol Bul. 1983 Oct;17(4):267-9.
5
Computer analysis of Staphylococcus aureus phage typing data from 1957 to 1975, citing epidemiological trends and natural evolution within the phage typing system.
Appl Environ Microbiol. 1980 Mar;39(3):623-9. doi: 10.1128/aem.39.3.623-629.1980.
6
LYSIS-FROM-WITHOUT OF STAPHYLOCOCCUS AUREUS STRAINS BY COMBINATIONS OF SPECIFIC PHAGES AND PHAGE-INDUCED LYTIC ENZYMES.
J Bacteriol. 1964 Sep;88(3):676-81. doi: 10.1128/jb.88.3.676-681.1964.
7
Phage typing of Staphylococcus aureus using phages other than those of basic set and new methods.
Indian J Med Res. 1982 Mar;75:348-54.
8
Phage-typing patterns and lysogenicity of methicillin-resistant strains of Staphylococcus aureus from Sydney, Australia, 1965-85.
J Med Microbiol. 1986 Nov;22(3):209-16. doi: 10.1099/00222615-22-3-209.
9
Staphylococcus in toxic shock syndrome and other surgical infections. Development of new bacteriophages.
Arch Surg. 1983 Mar;118(3):281-4. doi: 10.1001/archsurg.1983.01390030013002.
10
Lysogeny in staphylococci.
J Bacteriol. 1961 Dec;82(6):984-93. doi: 10.1128/jb.82.6.984-993.1961.
引用本文的文献
1
Bacteriophage therapy for multidrug-resistant infections: current technologies and therapeutic approaches.
J Clin Invest. 2025 Mar 3;135(5):e187996. doi: 10.1172/JCI187996.
2
Freeze-Drying of Encapsulated Bacteriophage T4 to Obtain Shelf-Stable Dry Preparations for Oral Application.
Pharmaceutics. 2023 Dec 17;15(12):2792. doi: 10.3390/pharmaceutics15122792.
3
Use of Trehalose as an Additive to Bacteriophage Vb_Pd_PDCC-1: Long-Term Preservation Analysis and Its Biocontrol Against Vibrio diabolicus Infection.
Curr Microbiol. 2023 Oct 16;80(12):372. doi: 10.1007/s00284-023-03487-7.
4
Essential Topics for the Regulatory Consideration of Phages as Clinically Valuable Therapeutic Agents: A Perspective from Spain.
Microorganisms. 2022 Mar 26;10(4):717. doi: 10.3390/microorganisms10040717.
5
Evaluation of the Stability of Bacteriophages in Different Solutions Suitable for the Production of Magistral Preparations in Belgium.
Viruses. 2021 May 8;13(5):865. doi: 10.3390/v13050865.
6
Stability of Staphylococcus aureus phage ISP after freeze-drying (lyophilization).
PLoS One. 2013 Jul 2;8(7):e68797. doi: 10.1371/journal.pone.0068797. Print 2013.
7
Phage pattern-specific oxacillin-resistant and borderline oxacillin-resistant Staphylococcus aureus in U.S. hospitals: epidemiological significance.
J Clin Microbiol. 1992 Jan;30(1):252-4. doi: 10.1128/jcm.30.1.252-254.1992.
本文引用的文献
1
A multiple-syringe bacteriophage applicator.
Am J Clin Pathol. 1960 Mar;33:233-7. doi: 10.1093/ajcp/33.3.233.
2
Preservation of staphylococcal bacteriophage by means of lyophilization.
Am J Clin Pathol. 1959 Apr;31(4):326-31. doi: 10.1093/ajcp/31.4.326.
3
Long-term Staphylococcus aureus carrier state in hospital patients.
J Clin Microbiol. 1982 Sep;16(3):517-20. doi: 10.1128/jcm.16.3.517-520.1982.
4
Computer analysis of Staphylococcus aureus phage typing data from 1957 to 1975, citing epidemiological trends and natural evolution within the phage typing system.
Appl Environ Microbiol. 1980 Mar;39(3):623-9. doi: 10.1128/aem.39.3.623-629.1980.
5
A hospital-wide outbreak of septicemia due to a few strains of Staphylococcus aureus.
Am J Clin Pathol. 1983 May;79(5):598-603. doi: 10.1093/ajcp/79.5.598.
6
Colonization of newly arrived house staff by virulent staphylococcal phage types endemic to a hospital environment.
J Clin Microbiol. 1986 Jun;23(6):1030-3. doi: 10.1128/jcm.23.6.1030-1033.1986.
Zotero 插件