Activation of muscle phosphofructokinase by alpha-glucose 1,6-bisphosphate and fructose 2,6-bisphosphate is differently affected by other allosteric effectors and by pH.

Citrate, ATP and AMP affect similarly the activation of muscle phosphofructokinase by alpha-glucose 1,6-bisphosphate and fructose 1,6-bisphosphate, but they affect differently its activation by fructose 2,6-bisphosphate. Activation by alpha-glucose 1,6-bisphosphate and fructose 2,6-bisphosphate is also differently affected by pH. This suggest that both alpha-glucose 1,6-bisphosphate and fructose 1,6-bisphosphate induce the same conformational change on muscle phosphofructokinase, distinct from that produced by fructose 2,6-bisphosphate.