Liang Min, Du Yuru, Li Wenjing, Yin Xi, Yang Ni, Qie Anran, Lebaron Tyler W, Zhang Jiayu, Chen Haiying, Shi Haishui
College of Nursing, Hebei Medical University.
Department of Biochemistry and Molecular Biology, College of Basic Medicine, Hebei Medical University.
Biol Pharm Bull. 2018 Jul 1;41(7):1040-1048. doi: 10.1248/bpb.b18-00082. Epub 2018 May 9.
SuHeXiang (SHX) has been used to treat a wide range of diseases, including those related to the central nervous system. However, the effects of SHX on mood disorders are still elusive. This study aimed to investigate the effects of SHX essential oil on stress-induced depression of mice. In an acute stress-induced depression model, mice inhaled vehicle (1% Tween 80) for 10 min or 10% SHX for 10 or 30 min once daily for 12 continuous days. In the chronic mild stress (CMS)-induced depression model, mice were exposed to a 28-d CMS treatment. Tail suspension test (TST), forced swimming test (FST), sucrose preference test (SPT), open field test (OFT), and novelty suppressed feeding (NSF) test were conducted. In addition, serum levels of angiogenin (ANG), thrombopoietin (TPO), interleukin 6 (IL-6), and tumor necrosis factor-α (TNF-α) were evaluated by enzyme-linked immunosorbent assay (ELISA) assays. The results showed that in mice exposed to acute stress, repeated SHX inhalation exerted significant antidepressant and anxiolytic activities, and also reduced the serum levels of ANG, TPO, IL-6, and TNF-α. It also significantly reversed the depressive and anxiety-like behaviors, and reduced the serum levels of ANG and TPO in mice exposed to CMS. This is the first report to show that SHX inhalation could produce significant antidepressant and anxiolytic-like effects. These effects might be mediated by SHX ability to modulate the inflammatory response, and reduce dysfunction of vascular genesis and thrombosis. These results support further exploration for developing SHX inhalation as a novel therapeutic strategy for depression and stress-related disorders.
苏合香（SHX）已被用于治疗多种疾病，包括与中枢神经系统相关的疾病。然而，SHX对情绪障碍的影响仍不明确。本研究旨在探讨SHX精油对小鼠应激诱导抑郁的影响。在急性应激诱导抑郁模型中，小鼠每天一次吸入赋形剂（1%吐温80）10分钟或10% SHX 10或30分钟，持续12天。在慢性轻度应激（CMS）诱导抑郁模型中，小鼠接受28天的CMS处理。进行了悬尾试验（TST）、强迫游泳试验（FST）、蔗糖偏好试验（SPT）、旷场试验（OFT）和新奇抑制摄食（NSF）试验。此外，通过酶联免疫吸附测定（ELISA）法评估血清血管生成素（ANG）、血小板生成素（TPO）、白细胞介素6（IL-6）和肿瘤坏死因子-α（TNF-α）水平。结果表明，在急性应激小鼠中，重复吸入SHX具有显著的抗抑郁和抗焦虑活性，还降低了血清ANG、TPO、IL-6和TNF-α水平。它还显著逆转了CMS小鼠的抑郁和焦虑样行为，并降低了血清ANG和TPO水平。这是首次报道吸入SHX可产生显著的抗抑郁和抗焦虑样效应。这些效应可能是由SHX调节炎症反应、减少血管生成和血栓形成功能障碍的能力介导的。这些结果支持进一步探索将吸入SHX作为治疗抑郁症和应激相关疾病的新型治疗策略。
