Liu Lianfang, Zhang Yonghua, Zhu Kai, Song Lili, Tao Minxian, Huang Pan, Pan Yingying
Department of Oncology, Zhangjiagang Hospital of Traditional Chinese Medicine affiliated to Nanjing University of Chinese Medicine, Jiangsu 215600, China.
J BUON. 2018 Mar-Apr;23(2):403-409.
To investigate the effect of resveratrol on the expression of leucine repeat immunoglobulin-like protein 1 (LRIG1) in glioma cell line U251 and the relationship between LRIG1 and U251 cell proliferation and apoptosis, so as to clarify other molecular mechanisms of resveratrol and look for possible new targets for the treatment of this condition.
U251 cells were treated with 100μM resveratrol for 48 hrs. Reverse transcription- polymerase chain reaction (RT-PCR) and Western blotting were used to detect the LRIG1 level in glioma cell line U251 and the expressions of related factors after resveratrol treatment. The loss-of-function assay was performed via transfection of LRIG1 small interference and the proliferation and apoptosis of U251 cells in each group were detected via MTT assay and flow cytometry.
The mRNA and protein expression levels of LRIG1 in U251 cells were up-regulated after resveratrol treatment, accompanied with decreased Epidermal Growth Factor Receptor (EGFR). MTT assay showed that the cell proliferation rate was decreased after resveratrol treatment, and flow cytometry showed that cell apoptosis was increased. The loss-of-function assay via transfection of LRIG1 small interference showed that resveratrol could reverse the increased cell proliferation and decreased apoptosis induced by LRIG1 small interference.
Resveratrol can inhibit the growth and proliferation of glioma and promote its apoptosis through upregulating the LRIG1 gene expression, which plays the effect of antiglioma growth, revealing that LRIG1 is a new biological target of resveratrol in antiglioma cell proliferation and growth.
探讨白藜芦醇对胶质瘤细胞系U251中亮氨酸重复免疫球蛋白样蛋白1（LRIG1）表达的影响，以及LRIG1与U251细胞增殖和凋亡的关系，以阐明白藜芦醇的其他分子机制，并寻找治疗该疾病可能的新靶点。
用100μM白藜芦醇处理U251细胞48小时。采用逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法检测胶质瘤细胞系U251中LRIG1水平以及白藜芦醇处理后相关因子的表达。通过转染LRIG1小干扰RNA进行功能缺失实验，并通过MTT法和流式细胞术检测每组U251细胞的增殖和凋亡情况。
白藜芦醇处理后，U251细胞中LRIG1的mRNA和蛋白表达水平上调，同时表皮生长因子受体（EGFR）表达降低。MTT法显示白藜芦醇处理后细胞增殖率降低，流式细胞术显示细胞凋亡增加。通过转染LRIG1小干扰RNA进行的功能缺失实验表明，白藜芦醇可逆转LRIG1小干扰RNA诱导的细胞增殖增加和凋亡减少。
白藜芦醇可通过上调LRIG1基因表达抑制胶质瘤的生长和增殖，并促进其凋亡，发挥抗胶质瘤生长的作用，提示LRIG1是白藜芦醇抗胶质瘤细胞增殖和生长的新生物学靶点。
