Resveratrol inhibits glioma cell growth via targeting LRIG1.

PURPOSE

To investigate the effect of resveratrol on the expression of leucine repeat immunoglobulin-like protein 1 (LRIG1) in glioma cell line U251 and the relationship between LRIG1 and U251 cell proliferation and apoptosis, so as to clarify other molecular mechanisms of resveratrol and look for possible new targets for the treatment of this condition.

METHODS

U251 cells were treated with 100μM resveratrol for 48 hrs. Reverse transcription- polymerase chain reaction (RT-PCR) and Western blotting were used to detect the LRIG1 level in glioma cell line U251 and the expressions of related factors after resveratrol treatment. The loss-of-function assay was performed via transfection of LRIG1 small interference and the proliferation and apoptosis of U251 cells in each group were detected via MTT assay and flow cytometry.

RESULTS

The mRNA and protein expression levels of LRIG1 in U251 cells were up-regulated after resveratrol treatment, accompanied with decreased Epidermal Growth Factor Receptor (EGFR). MTT assay showed that the cell proliferation rate was decreased after resveratrol treatment, and flow cytometry showed that cell apoptosis was increased. The loss-of-function assay via transfection of LRIG1 small interference showed that resveratrol could reverse the increased cell proliferation and decreased apoptosis induced by LRIG1 small interference.

CONCLUSIONS

Resveratrol can inhibit the growth and proliferation of glioma and promote its apoptosis through upregulating the LRIG1 gene expression, which plays the effect of antiglioma growth, revealing that LRIG1 is a new biological target of resveratrol in antiglioma cell proliferation and growth.