Department of Animal Science, University of Sao Paulo, Pirassununga, Sao Paulo 13635-015, Brazil.
J Anim Sci. 2018 Jun 4;96(6):2371-2379. doi: 10.1093/jas/sky062.
The study was conducted to determine the effects of multicarbohydrase (MC) preparation (700 U α-galactosidase, 2,200 U galactomannanase, 3,000 U xylanase, and 22,000 U β-glucanase per kg of diet) and phytase (Phy, 500 FTU per kg of diet) supplementation on the nutritive value of wheat bran (WB) in broiler chicks. Trial 1 determined retention of nutrients and apparent metabolizable energy corrected by nitrogen (AMEn). One reference diet (RD) protein-free (85% corn based) was fortified to determine the WB nutrient retention coefficient. Trial 2 determined standardized ileal digestibility (SID) of AA, when pancreas and liver were weighed. An additional group of bird was fed with an RD with 5% casein-corn starch diet, fortified with vitamins and minerals to quantify the endogenous fraction and determine SID of AA. For each trial, the test diets were made by mixing RD and WB 7:3 (wt/wt) and fed without or with MC or Phy or combination. Male broilers (Cobb 500), 245 d old, were allocated to five treatments to give seven replicates (seven birds/cage). The birds were fed a commercial diet from day 0 to10 followed by Trial 1 diets from day 11 to 18 and finally Trial 2 diets from day 19 to 21. Excreta samples were collected on days 15-18 and all birds were slaughtered on day 21 for ileal digesta. There was an interaction (P < 0.05) between MC and Phy on retention of DM, N, P, and AMEn. An interaction (P < 0.05) was also observed on SID of Arg, His, Leu, Lys, Phe, Thr, Val, Asp, Cys, Glu, and Ser. Responses of MC plus Phy supplementation were higher (P < 0.05) on overall SID of AA by 6.05% (75.18 to 94.26%), compared with responses for MC (2.35%; 72.04 to 88.97) or Phy (3.46%; 73.27 to 92.13). Liver and pancreas weights were affected (P < 0.05) by the single MC supplementation. The MC and Phy combination may be an effective strategy to improve AA utilization of WB in broiler chickens.
该研究旨在确定多聚糖酶（MC）制剂（700U α-半乳糖苷酶、2200U 半乳甘露聚糖酶、3000U 木聚糖酶和 22000U β-葡聚糖酶/千克日粮）和植酸酶（Phy，500FTU/千克日粮）对肉鸡小麦麸营养价值的影响。试验 1 确定了养分和经氮校正的表观代谢能（AMEn）的保留率。一种参考无蛋白日粮（RD）（85%基于玉米）被强化，以确定小麦麸的养分保留系数。试验 2 确定了当胰腺和肝脏称重时，AA 的标准回肠可消化性（SID）。另外一组鸡饲喂含有 5%酪蛋白-玉米淀粉日粮的 RD，该日粮添加了维生素和矿物质，以量化内源性部分，并确定 AA 的 SID。对于每个试验，试验日粮都是通过将 RD 和小麦麸以 7:3（重量/重量）混合制成，并在没有 MC 或 Phy 或两者组合的情况下进行喂养。245 日龄的雄性肉鸡（Cobb 500）被分配到五个处理组，每个处理组 7 个重复（每个笼子 7 只鸡）。这些鸡从第 0 天到第 10 天饲喂商业日粮，然后从第 11 天到第 18 天饲喂试验 1 日粮，最后从第 19 天到第 21 天饲喂试验 2 日粮。第 15-18 天收集排泄物样本，第 21 天所有鸡均屠宰，用于回肠内容物。MC 和 Phy 之间存在互作（P＜0.05），影响 DM、N、P 和 AMEn 的保留率。Arg、His、Leu、Lys、Phe、Thr、Val、Asp、Cys、Glu 和 Ser 的 SID 也存在互作（P＜0.05）。与 MC（2.35%；72.04 至 88.97）或 Phy（3.46%；73.27 至 92.13）的反应相比，MC 加 Phy 补充的反应更高（P＜0.05），整体 AA 的 SID 提高了 6.05%（75.18 至 94.26%）。MC 单一补充会影响肝脏和胰腺的重量（P＜0.05）。MC 和 Phy 联合使用可能是提高肉鸡小麦麸 AA 利用率的有效策略。
