Department of Animal Nutrition and Production, University of Sao Paulo, Pirassununga, Sao Paulo, Brazil.
Department of Animal Biosciences, University of Guelph, Guelph, ON, Canada.
Poult Sci. 2017 Sep 1;96(9):3305-3313. doi: 10.3382/ps/pex141.
Two assays were conducted to evaluate nutritive value of canola meal (CM) fed to broiler chicks without or with a multi-carbohydrase (MC) preparation (700 U α-galactosidase, 2,200 U galactomannanase, 30,000 U xylanase, and 22,000 U β-glucanase per kg of diet) and phytase (Phy, 500 FTU per kg of diet). Assay 1 determined apparent total tract digestibility (ATTD) of nutrients and metabolizable energy (AME) by the difference method. Assay 2 determined apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids by the index method. Two reference diets (RD) - an 85% corn based and a 5% casein-cornstarch diet fortified with vitamins and minerals - were made for assays 1 and 2, respectively. For each assay, the test diets were made by mixing RD and CM 7:3 wt/wt basis and fed without or with MC or Phy or combination. A total of 245 day-old male broilers (Cobb 500) was allocated to 5 treatments to give 7 replicates (7 birds/cage). The birds were fed a commercial diet from day zero to 10 followed by assay 1 fed from d 11 to 18 and assay 2 fed from d 19 to 21. Excreta samples were collected on d 15 to18, and all birds were slaughtered on d 21 for ileal digesta. There was an interaction (P < 0.05) between MC and Phy on ATTD of DM, N, and P. There was no interaction (P > 0.05) between MC and Phy on AMEn; however, MC and Phy individually improved AMEn retention. Enzymes interacted (P < 0.05) on SID of Arg, His, Leu, Met, Thr, Ala, Asp, Gln, and Gly. In this context, feeding a combination of MC and Phy resulted in higher (P < 0.05) SID of Arg, His, Met, and Thr relative to single activity or control. Both enzymes improved (P < 0.05) SID of Lys independently. The combination of carbohydrase and Phy may be an effective strategy to improve amino acid utilization in CM for poultry.
进行了两项试验来评估菜籽粕（CM）在没有或添加多酶（MC，700 U α-半乳糖苷酶、2200 U 甘露聚糖酶、30000 U 木聚糖酶和 22000 U β-葡聚糖酶/千克日粮）和植酸酶（Phy，500 FTU/千克日粮）条件下对肉鸡的营养价值。试验 1 通过差减法测定养分和代谢能（AME）的全肠道表观消化率（ATTD）。试验 2 通过指数法测定氨基酸的回肠表观消化率（AID）和标准回肠可消化率（SID）。为试验 1 和 2 分别制作了两种参考日粮（RD）-85%玉米基础日粮和 5%酪蛋白-玉米淀粉日粮，并用维生素和矿物质强化。对于每个试验，试验日粮是通过将 RD 和 CM 以 7:3（重量/重量）的基础混合并添加或不添加 MC 或 Phy 或两者的组合制成的。共 245 只 1 日龄雄性肉鸡（科宝 500）被分配到 5 种处理中，每个处理 7 个重复（每个鸡笼 7 只鸡）。鸡从 0 日龄开始饲喂商业日粮，然后进行试验 1，从第 11 天到第 18 天，试验 2 从第 19 天到第 21 天。在第 15 天到 18 天收集排泄物样品，所有鸡在第 21 天屠宰，用于回肠食糜。MC 和 Phy 对 DM、N 和 P 的 ATTD 存在互作（P<0.05）。MC 和 Phy 对 AMEn 无互作（P>0.05）；然而，MC 和 Phy 各自提高了 AMEn 的保留率。酶之间存在互作（P<0.05），影响 Arg、His、Leu、Met、Thr、Ala、Asp、Gln 和 Gly 的 SID。在这种情况下，与单一活性或对照相比，同时添加 MC 和 Phy 可提高 Arg、His、Met 和 Thr 的 SID（P<0.05）。两种酶均独立提高（P<0.05）Lys 的 SID。在这种情况下，添加碳水化合物酶和植酸酶可能是提高家禽菜籽粕中氨基酸利用率的有效策略。
