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利用β-半乳糖苷酶包覆的磁性纳米颗粒检测水中的细菌。

Detection of Bacteria in Water with β-Galactosidase-Coated Magnetic Nanoparticles.

机构信息

1 Nanyang Environment & Water Research Institute, Interdisciplinary Graduate School, Nanyang Technological University, Singapore.

2 School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.

出版信息

SLAS Technol. 2018 Dec;23(6):624-630. doi: 10.1177/2472630318773407. Epub 2018 May 10.

DOI:10.1177/2472630318773407
PMID:29746796
Abstract

Pathogenic contamination and resistant bacterial infections remain critical concerns in both developed and developing countries. Rapid and sensitive detection of pathogens is still a key requirement for both environmental and clinical settings. This article introduces a simple, colorimetric, cost-effective, and high-throughput system based on a positively charged iron oxide/enzyme complex for the detection of both gram-positive and gram-negative bacteria in water between 10 and 10 cfu/mL. This study provides an effective strategy for the identification and purification of pathogen contamination in drinking water.

摘要

在发达国家和发展中国家,病原污染和耐药菌感染仍然是人们关注的焦点。快速、灵敏地检测病原体仍然是环境和临床环境的关键要求。本文介绍了一种简单、比色、经济高效、高通量的系统,该系统基于带正电荷的氧化铁/酶复合物,用于检测水介质中 10 至 10 cfu/mL 之间的革兰氏阳性菌和革兰氏阴性菌。本研究为鉴定和净化饮用水中的病原体污染提供了一种有效的策略。

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