Conti G, Portincasa P, Chezzi C, Sanna A
Instituto di Microbiologia, Facoltà di Medicina e Chirurgia, Università delgi Studi, Parma, Italy.
Ann Inst Pasteur Virol. 1988 Jan-Mar;139(1):69-78. doi: 10.1016/s0769-2617(88)80007-8.
The growth cycle of influenza virus strain FPV, Ulster 73, was altered by treatment of LLC-MK2 cells with diamidinophenylindole. Viral protein synthesis was restricted to the early pattern of virus multiplication, and post-treatment experiments showed the ability of the drug to block virus replication until the 4th hour p.i. Drug addition (followed by removal) revealed the inhibition of synthesis of late viral products, and especially of membrane protein. Kinetic studies on the production of viral RNA indicated a decrease in the synthesis of late virus-induced RNA species, suggesting that the target of DAPI is probably the late transcription of the virus genome. The nonpermissive condition mediated by the drug could represent a suitable model to study cellular intervention during viral growth.
用双脒基苯基吲哚处理LLC - MK2细胞后，流感病毒株FPV、阿尔斯特73的生长周期发生了改变。病毒蛋白合成被限制在病毒增殖的早期模式，治疗后实验表明该药物能够在感染后第4小时之前阻断病毒复制。添加（随后去除）药物显示出对晚期病毒产物合成的抑制作用，尤其是对膜蛋白的抑制。对病毒RNA产生的动力学研究表明，晚期病毒诱导的RNA种类的合成减少，这表明DAPI的靶标可能是病毒基因组的晚期转录。药物介导的非允许条件可能是研究病毒生长过程中细胞干预的合适模型。
