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甲型流感病毒NS 2非结构蛋白信使核糖核酸的合成在没有病毒蛋白合成的情况下发生。

Synthesis of the NS 2 nonstructural protein messenger RNA of influenza A viruses occurs in the absence of viral protein synthesis.

作者信息

Odagiri T, Tobita K, Tashiro M

机构信息

Department of Virology, Jichi Medical School, Tochigi-ken, Japan.

出版信息

Arch Virol. 1991;120(3-4):281-8. doi: 10.1007/BF01310483.

DOI:10.1007/BF01310483
PMID:1835573
Abstract

The NS 2 messenger RNA (mRNA) of several influenza A viruses was shown to be synthesized in primary transcription. Analysis of in vitro translation products of mRNAs from infected MDCK cells treated with cycloheximide indicated that the NS 2 mRNA in addition to the NS 1 mRNA was synthesized with PR/8, Udorn, and Aichi viruses. The findings indicated that the NS 1 mRNA of these viruses was able to be spliced into the NS 2 mRNA as a primary transcript without viral protein synthesis, although the extent of splicing varied among different virus strains.

摘要

几种甲型流感病毒的NS 2信使核糖核酸（mRNA）在初级转录过程中被合成。对用环己酰亚胺处理的感染MDCK细胞的mRNA体外翻译产物的分析表明，除了NS 1 mRNA外，NS 2 mRNA也与PR/8、乌栋和爱知病毒一起被合成。研究结果表明，这些病毒的NS 1 mRNA能够作为初级转录本剪接成NS 2 mRNA，而无需病毒蛋白合成，尽管不同病毒株的剪接程度有所不同。

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本文引用的文献

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Sequence of interrupted and uninterrupted mRNAs and cloned DNA coding for the two overlapping nonstructural proteins of influenza virus.流感病毒两种重叠非结构蛋白的间断和不间断mRNA序列以及编码它们的克隆DNA
Cell. 1980 Sep;21(2):475-85. doi: 10.1016/0092-8674(80)90484-5.
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Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins.源自流感病毒基因组RNA片段7的mRNA序列：共线性和间断性mRNA编码重叠蛋白。
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Differences in the control of virus mRNA splicing during permissive or abortive infection with influenza A (fowl plague) virus.
甲型流感（禽瘟）病毒允许性感染或流产性感染期间病毒mRNA剪接控制的差异。
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Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli.流感病毒NS1非结构蛋白在大肠杆菌中的高效表达。
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Mapping of the two overlapping genes for polypeptides NS1 and NS2 on RNA segment 8 of influenza virus genome.流感病毒基因组第8个RNA片段上编码NS1和NS2多肽的两个重叠基因的定位
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Spliced and unspliced messenger RNAs synthesized from cloned influenza virus M DNA in an SV40 vector: expression of the influenza virus membrane protein (M1).从SV40载体中克隆的流感病毒M DNA合成的剪接和未剪接信使RNA：流感病毒膜蛋白（M1）的表达
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Regulated production of an influenza virus spliced mRNA mediated by virus-specific products.由病毒特异性产物介导的流感病毒剪接mRNA的调控产生。
EMBO J. 1985 Sep;4(9):2313-9. doi: 10.1002/j.1460-2075.1985.tb03932.x.
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Temperature-sensitive defect of influenza A/Ann Arbor/6/60 cold-adapted variant leads to a blockage of matrix polypeptide incorporation into the plasma membrane of the infected cells.
Virus Res. 1987 May;7(3):203-18. doi: 10.1016/0168-1702(87)90028-1.
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In vitro splicing of influenza viral NS1 mRNA and NS1-beta-globin chimeras: possible mechanisms for the control of viral mRNA splicing.流感病毒NS1 mRNA和NS1-β-珠蛋白嵌合体的体外剪接：病毒mRNA剪接调控的可能机制
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5444-8. doi: 10.1073/pnas.83.15.5444.
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