Conti G, Portincasa P
Arch Virol. 1987;94(1-2):149-57. doi: 10.1007/BF01313733.
The production of avian and human strains of influenza virus was altered to various extents by treatment of various host cells with 40 micrograms/ml of diamidinophenylindole (DAPI). In infected LLC-MK 2 cells only an abortive replication cycle occurred; in other cell lines there was partial inhibition or no inhibition of replication. Virus polypeptide synthesis in LLC-MK 2 cells was confined to the early pattern of viral multiplication; only the P proteins, the nucleoprotein NP, and the non-structural protein NS 1 were synthesized. The stage of replication mainly affected by DAPI was between the fourth and the sixth hour after infection. The mode of action of the drug and its modulating effect on virus production is discussed.
用40微克/毫升的二脒基苯基吲哚（DAPI）处理各种宿主细胞，不同程度地改变了禽流感病毒和人流感病毒株的产生。在感染的LLC-MK 2细胞中，只出现了一个流产的复制周期；在其他细胞系中，复制受到部分抑制或没有抑制。LLC-MK 2细胞中的病毒多肽合成局限于病毒增殖的早期模式；只合成了P蛋白、核蛋白NP和非结构蛋白NS 1。主要受DAPI影响的复制阶段是在感染后第四至第六小时之间。讨论了该药物的作用方式及其对病毒产生的调节作用。
