• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

植物内体运输中两个 RAB5 小 GTPase 家族的整合。

Integration of two RAB5 groups during endosomal transport in plants.

机构信息

Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.

Department of Natural Sciences, International Christian University, Tokyo, Japan.

出版信息

Elife. 2018 May 11;7:e34064. doi: 10.7554/eLife.34064.

DOI:10.7554/eLife.34064
PMID:29749929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5947987/
Abstract

RAB5 is a key regulator of endosomal functions in eukaryotic cells. Plants possess two different RAB5 groups, canonical and plant-unique types, which act via unknown counteracting mechanisms. Here, we identified an effector molecule of the plant-unique RAB5 in , ARA6, which we designated PLANT-UNIQUE RAB5 EFFECTOR 2 (PUF2). Preferential colocalization with canonical RAB5 on endosomes and genetic interaction analysis indicated that PUF2 coordinates vacuolar transport with canonical RAB5, although PUF2 was identified as an effector of ARA6. Competitive binding of PUF2 with GTP-bound ARA6 and GDP-bound canonical RAB5, together interacting with the shared activating factor VPS9a, showed that ARA6 negatively regulates canonical RAB5-mediated vacuolar transport by titrating PUF2 and VPS9a. These results suggest a unique and unprecedented function for a RAB effector involving the integration of two RAB groups to orchestrate endosomal trafficking in plant cells.

摘要

RAB5 是真核细胞内内体功能的关键调节因子。植物拥有两种不同的 RAB5 群，即经典和植物特有的类型，它们通过未知的拮抗机制发挥作用。在这里，我们鉴定了植物特有的 RAB5 的一个效应分子，ARA6，我们将其命名为植物特有的 RAB5 效应子 2（PUF2）。优先与内体上的经典 RAB5 共定位以及遗传相互作用分析表明，PUF2 与经典 RAB5 一起协调液泡运输，尽管 PUF2 被鉴定为 ARA6 的效应子。PUF2 与 GTP 结合的 ARA6 和 GDP 结合的经典 RAB5 的竞争性结合，以及与共享激活因子 VPS9a 的相互作用表明，ARA6 通过滴定 PUF2 和 VPS9a 负调节经典 RAB5 介导的液泡运输。这些结果表明，一种涉及两个 RAB 群整合以协调植物细胞内体运输的 RAB 效应子具有独特的、前所未有的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/6ce66ba07377/elife-34064-fig7-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/1c6cf902c5a8/elife-34064-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/4fe702533e6d/elife-34064-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/a6ea904546b1/elife-34064-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/12bb0b47cd87/elife-34064-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/c2a2d97918f4/elife-34064-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/c744f6320159/elife-34064-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/0308029f4439/elife-34064-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/685085500682/elife-34064-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/5385b2d66e86/elife-34064-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/c40c826a127c/elife-34064-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/6f9733937fe3/elife-34064-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/bd3a30ee2f72/elife-34064-fig6-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/125ed5854617/elife-34064-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/6ce66ba07377/elife-34064-fig7-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/1c6cf902c5a8/elife-34064-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/4fe702533e6d/elife-34064-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/a6ea904546b1/elife-34064-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/12bb0b47cd87/elife-34064-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/c2a2d97918f4/elife-34064-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/c744f6320159/elife-34064-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/0308029f4439/elife-34064-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/685085500682/elife-34064-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/5385b2d66e86/elife-34064-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/c40c826a127c/elife-34064-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/6f9733937fe3/elife-34064-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/bd3a30ee2f72/elife-34064-fig6-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/125ed5854617/elife-34064-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/5947987/6ce66ba07377/elife-34064-fig7-figsupp1.jpg

相似文献

1
Integration of two RAB5 groups during endosomal transport in plants.植物内体运输中两个 RAB5 小 GTPase 家族的整合。
Elife. 2018 May 11;7:e34064. doi: 10.7554/eLife.34064.
2
Functional analyses of the plant-specific C-terminal region of VPS9a: the activating factor for RAB5 in Arabidopsis thaliana.拟南芥中VPS9a植物特异性C末端区域的功能分析：RAB5的激活因子
J Plant Res. 2016 Jan;129(1):93-102. doi: 10.1007/s10265-015-0760-5.
3
ENDOSOMAL RAB EFFECTOR WITH PX-DOMAIN, an Interacting Partner of RAB5 GTPases, Regulates Membrane Trafficking to Protein Storage Vacuoles in Arabidopsis.具有PX结构域的内体RAB效应蛋白，RAB5 GTP酶的相互作用伴侣，调节拟南芥中向蛋白质储存液泡的膜运输。
Plant Cell. 2016 Jun;28(6):1490-503. doi: 10.1105/tpc.16.00326. Epub 2016 Jun 10.
4
Purification and Interaction Analysis of a Plant-Specific RAB5 Effector by In Vitro Pull-Down Assay.利用体外下拉实验对一种植物特异性 RAB5 效应物进行纯化和相互作用分析。
Methods Mol Biol. 2020;2177:183-197. doi: 10.1007/978-1-0716-0767-1_15.
5
Modulation of Plant RAB GTPase-Mediated Membrane Trafficking Pathway at the Interface Between Plants and Obligate Biotrophic Pathogens.植物与专性活体营养型病原菌界面处植物RAB GTP酶介导的膜运输途径的调控
Plant Cell Physiol. 2016 Sep;57(9):1854-64. doi: 10.1093/pcp/pcw107. Epub 2016 Jun 18.
6
VPS9a, the common activator for two distinct types of Rab5 GTPases, is essential for the development of Arabidopsis thaliana.VPS9a是两种不同类型的Rab5 GTP酶的共同激活剂，对拟南芥的发育至关重要。
Plant Cell. 2007 Nov;19(11):3504-15. doi: 10.1105/tpc.107.053876. Epub 2007 Nov 30.
7
The Plant-Specific RAB5 GTPase ARA6 is Required for Starch and Sugar Homeostasis in Arabidopsis thaliana.拟南芥中淀粉和糖稳态需要植物特有的RAB5 GTP酶ARA6 。
Plant Cell Physiol. 2015 Jun;56(6):1073-83. doi: 10.1093/pcp/pcv029. Epub 2015 Feb 23.
8
RAB5 activation is required for multiple steps in Arabidopsis thaliana root development.RAB5 的激活对于拟南芥根发育的多个步骤是必需的。
Plant Cell Physiol. 2013 Oct;54(10):1648-59. doi: 10.1093/pcp/pct109. Epub 2013 Jul 27.
9
A membrane trafficking pathway regulated by the plant-specific RAB GTPase ARA6.一种由植物特异性 RAB GTPase ARA6 调控的膜运输途径。
Nat Cell Biol. 2011 Jun 12;13(7):853-9. doi: 10.1038/ncb2270.
10
Arabidopsis sterol endocytosis involves actin-mediated trafficking via ARA6-positive early endosomes.拟南芥固醇内吞作用涉及通过ARA6阳性早期内体进行的肌动蛋白介导的运输。
Curr Biol. 2003 Aug 19;13(16):1378-87. doi: 10.1016/s0960-9822(03)00538-4.

引用本文的文献

1
The Golgi Apparatus and its Next-Door Neighbors.高尔基体及其近邻
Front Cell Dev Biol. 2022 Apr 28;10:884360. doi: 10.3389/fcell.2022.884360. eCollection 2022.
2
RAB GTPases and SNAREs at the trans-Golgi network in plants.植物中转高尔基网络中的 RAB GTPases 和 SNAREs。
J Plant Res. 2022 May;135(3):389-403. doi: 10.1007/s10265-022-01392-x. Epub 2022 Apr 29.
3
Plant RABs: Role in Development and in Abiotic and Biotic Stress Responses.植物RAB蛋白：在发育以及非生物和生物胁迫响应中的作用

本文引用的文献

1
Distinct sets of tethering complexes, SNARE complexes, and Rab GTPases mediate membrane fusion at the vacuole in Arabidopsis.在拟南芥中，不同的系绳复合物、SNARE 复合物和 Rab GTPases 介导液泡的膜融合。
Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):E2457-E2466. doi: 10.1073/pnas.1717839115. Epub 2018 Feb 20.
2
Modulation of Plant RAB GTPase-Mediated Membrane Trafficking Pathway at the Interface Between Plants and Obligate Biotrophic Pathogens.植物与专性活体营养型病原菌界面处植物RAB GTP酶介导的膜运输途径的调控
Plant Cell Physiol. 2016 Sep;57(9):1854-64. doi: 10.1093/pcp/pcw107. Epub 2016 Jun 18.
3
Curr Genomics. 2021 Jan;22(1):26-40. doi: 10.2174/1389202922666210114102743.
4
Salt-induced expression of intracellular vesicle trafficking genes, CaRab-GTP, and their association with Na accumulation in leaves of chickpea (Cicer arietinum L.).盐诱导的细胞内囊泡运输基因 CaRab-GTP 的表达及其与鹰嘴豆（Cicer arietinum L.）叶片中 Na 积累的关系。
BMC Plant Biol. 2020 Oct 14;20(Suppl 1):183. doi: 10.1186/s12870-020-02331-5.
5
A Conditional Mutation in Reveals Linkage Between PIN Protein Trafficking, Auxin Transport, Gravitropism, and Lateral Root Initiation.一个条件性突变揭示了PIN蛋白运输、生长素运输、向重力性和侧根起始之间的联系。
Front Plant Sci. 2020 Jul 7;11:910. doi: 10.3389/fpls.2020.00910. eCollection 2020.
6
Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation among Fruit Tissues in Late Development.转录组和激素分析揭示了晚期果实组织中激素和囊泡运输调控的见解。
Int J Mol Sci. 2020 Jul 8;21(14):4819. doi: 10.3390/ijms21144819.
7
Zipcode RNA-Binding Proteins and Membrane Trafficking Proteins Cooperate to Transport Glutelin mRNAs in Rice Endosperm.Zipcode RNA 结合蛋白和膜转运蛋白在水稻胚乳中合作运输谷蛋白 mRNAs。
Plant Cell. 2020 Aug;32(8):2566-2581. doi: 10.1105/tpc.20.00111. Epub 2020 May 29.
8
MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway.MTV 蛋白揭示了植物液泡运输途径中内质网和微管相关的隔室。
Proc Natl Acad Sci U S A. 2020 May 5;117(18):9884-9895. doi: 10.1073/pnas.1919820117. Epub 2020 Apr 22.
9
Remove, Recycle, Degrade: Regulating Plasma Membrane Protein Accumulation.去除、回收、降解：调控质膜蛋白积累。
Plant Cell. 2019 Dec;31(12):2833-2854. doi: 10.1105/tpc.19.00433. Epub 2019 Oct 18.
ENDOSOMAL RAB EFFECTOR WITH PX-DOMAIN, an Interacting Partner of RAB5 GTPases, Regulates Membrane Trafficking to Protein Storage Vacuoles in Arabidopsis.
具有PX结构域的内体RAB效应蛋白，RAB5 GTP酶的相互作用伴侣，调节拟南芥中向蛋白质储存液泡的膜运输。
Plant Cell. 2016 Jun;28(6):1490-503. doi: 10.1105/tpc.16.00326. Epub 2016 Jun 10.
4
Multiple Types of Guanine Nucleotide Exchange Factors (GEFs) for Rab Small GTPases.Rab小GTP酶的多种类型鸟嘌呤核苷酸交换因子（GEFs）
Cell Struct Funct. 2016 Jul 9;41(2):61-79. doi: 10.1247/csf.16008. Epub 2016 May 27.
5
Functional analyses of the plant-specific C-terminal region of VPS9a: the activating factor for RAB5 in Arabidopsis thaliana.拟南芥中VPS9a植物特异性C末端区域的功能分析：RAB5的激活因子
J Plant Res. 2016 Jan;129(1):93-102. doi: 10.1007/s10265-015-0760-5.
6
SMART: recent updates, new developments and status in 2015.SMART：2015年的近期更新、新进展及现状
Nucleic Acids Res. 2015 Jan;43(Database issue):D257-60. doi: 10.1093/nar/gku949. Epub 2014 Oct 9.
7
Plant vacuolar trafficking occurs through distinctly regulated pathways.植物液泡运输通过明显受调控的途径发生。
Curr Biol. 2014 Jun 16;24(12):1375-1382. doi: 10.1016/j.cub.2014.05.004. Epub 2014 May 29.
8
Protein delivery to vacuole requires SAND protein-dependent Rab GTPase conversion for MVB-vacuole fusion.蛋白质输送至液泡需要依赖SAND蛋白的Rab GTP酶转换以实现多泡体与液泡融合。
Curr Biol. 2014 Jun 16;24(12):1383-1389. doi: 10.1016/j.cub.2014.05.005. Epub 2014 May 29.
9
RAB5 activation is required for multiple steps in Arabidopsis thaliana root development.RAB5 的激活对于拟南芥根发育的多个步骤是必需的。
Plant Cell Physiol. 2013 Oct;54(10):1648-59. doi: 10.1093/pcp/pct109. Epub 2013 Jul 27.
10
Fiji: an open-source platform for biological-image analysis.斐济：一个用于生物影像分析的开源平台。
Nat Methods. 2012 Jun 28;9(7):676-82. doi: 10.1038/nmeth.2019.