Simplot Plant Sciences, J.R. Simplot Company, Boise, ID, USA.
Simplot Plant Sciences, J.R. Simplot Company, Boise, ID, USA.
Food Chem. 2018 Oct 1;262:191-198. doi: 10.1016/j.foodchem.2018.04.051. Epub 2018 Apr 16.
Sucrose and its constituent components, glucose and fructose, are major determinants of sweetness in fruits and cooking quality in plant storage organs, such as potato tubers. Accurate monitoring of these sugars provides a predictive assessment of plant product quality. High Performance Liquid Chromatography (HPLC) coupled to a variety of detectors has been the method of choice for selective detection and quantitation of sugars in plant tissues. However, sugar extraction can be time-consuming to obtain a clean and concentrated sample suitable for accurate quantitation. Herein, we took advantage of a modern HPLC system coupled to a highly sensitive high-resolution high-accuracy mass spectrometer to develop a rapid method for measuring glucose, fructose, and sucrose in plant tissues. The method was validated in mature potato tubers and strawberry fruits and it proved to be highly accurate and robust with minimal sample care requirements.
蔗糖及其组成成分葡萄糖和果糖是水果甜度和植物储存器官（如土豆块茎）烹饪品质的主要决定因素。这些糖的准确监测可对植物产品质量进行预测评估。高效液相色谱法（HPLC）结合多种检测器一直是植物组织中糖的选择性检测和定量的首选方法。然而，为了获得适合准确定量的清洁且浓缩的样品，糖提取可能很耗时。在此，我们利用现代 HPLC 系统结合高灵敏度、高分辨率、高精度质谱仪开发了一种快速测量植物组织中葡萄糖、果糖和蔗糖的方法。该方法在成熟的土豆块茎和草莓果实中进行了验证，结果表明该方法具有高度准确性和稳健性，对样品处理要求最低。
