College of Life Sciences, Henan Agricultural University, Zhengzhou, China.
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Methods Mol Biol. 2024;2830:73-80. doi: 10.1007/978-1-0716-3965-8_7.
Raffinose family oligosaccharides (RFOs) are synthesized from sucrose and subsequent addition of galactose moieties which was provided by galactinol. Galactinol is synthesized from UDP-galactose and myo-inositol. RFOs accumulate at late stage of seed development and play important roles in seed longevity. RFOs are major components in seeds of many plant species. Here, we document a methodology for extraction and quantitative analysis of raffinose metabolism-related soluble sugars or the derivative alcohols in plant seeds. This protocol, based on high-performance liquid chromatography (HPLC), achieves the efficient separation and accurate quantification of sucrose, myo-inositol, galactinol, and raffinose within 25 min of retention time.
棉子糖家族低聚糖（RFOs）是由蔗糖合成的，随后添加半乳糖残基，而半乳糖残基是由半乳糖醇提供的。半乳糖醇是由 UDP-半乳糖和肌醇合成的。RFOs 在种子发育后期积累，在种子寿命中发挥重要作用。RFOs 是许多植物种子的主要成分。在这里，我们记录了一种从植物种子中提取和定量分析棉子糖代谢相关可溶性糖或其衍生醇的方法。该方案基于高效液相色谱（HPLC），可在 25 分钟的保留时间内实现蔗糖、肌醇、半乳糖醇和棉子糖的高效分离和准确定量。
