Khan Anwar Sheed, Ali Sajid, Khan Muhammad Tahir, Ahmed Sajjad, Khattak Yasir, Irfan Muhammad, Sajjad Wasim
Khyber Medical University Peshawar, Peshawar, Pakistan.
Provincial TB Reference Laboratory, Peshawar, Pakistan.
Braz J Microbiol. 2018 Oct-Dec;49(4):909-913. doi: 10.1016/j.bjm.2018.02.011. Epub 2018 Apr 27.
GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n=390) to 47% (n=347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40-50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy.
GeneXpert是近期用于在短时间内诊断结核病的技术仪器之一。在本研究中,在巴基斯坦开伯尔-普赫图赫瓦省,对GeneXpert MTB/RIF检测法诊断肺外结核病(EPTB)的性能与发光二极管荧光显微镜检查(LED-FM)进行了比较。共从疑似结核病(TB)患者中收集了737份EPTB样本。在这些样本中,男性与女性的比例分别为53%(n = 390)和47%(n = 347)。GeneXpert的灵敏度和特异性分别为73%和100%,而LED-FM显微镜检查的灵敏度和特异性分别为40%和100%。这表明GeneXpert的灵敏度比LED-FM显微镜检查高40 - 50%。与LED-FM显微镜检查相比,GeneXpert检测到的杆菌数量也较少。