Department of Chemical Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700 009, West Bengal, India.
ICMR Virus Unit, I.D. and B.G. Hospital, General Block 4, First Floor, 57 Dr Suresh C Banerjee Road, Beliaghata, Kolkata 700 010, West Bengal, India.
Mater Sci Eng C Mater Biol Appl. 2018 Aug 1;89:413-421. doi: 10.1016/j.msec.2018.04.005. Epub 2018 Apr 5.
This work relates to quasi spherical gold nanoparticles synthesis and successful antiviral efficacy evaluations against Herpes simplex virus (HSV) infections. Ultrasound induced rapid reduction in gallic acid (GA) leads to highly monodispersed gold nanoparticles (GAunps). GAunps plasmonic peak was recorded at 531 nm with TEM size of 7.86 nm. X-ray diffraction and SAED pattern proved fcc crystalline structure. FTIR studies confirmed nanoparticles surface conjugation with gallic acid. The antiviral efficacy of GAunps was studied against HSV infections in Vero cells. GAunps were effective in dose-dependent manner with EC of 32.3 μM in HSV-1 and 38.6 μM in HSV-2. Further study revealed that GAunps prevented viral attachment and penetration into the Vero cells. The inhibition percentage varied with the nanoparticles exposure time in infected cells. Nanoparticles cytotoxicity (CC 972.4 μM) in Vero cells was significantly lower than acyclovir (CC 561.7 μM) indicating its safety. Bio-safe gold nanoparticles were proposed as a safer alternative in virus chemotherapy.
这项工作涉及到准球形金纳米粒子的合成，并成功评估了其针对单纯疱疹病毒 (HSV) 感染的抗病毒功效。超声诱导的没食子酸 (GA) 快速还原导致了高度单分散的金纳米粒子 (GAunps)。GAunps 的等离子体峰在 531nm 处被记录，TEM 尺寸为 7.86nm。X 射线衍射和 SAED 图案证明了 fcc 晶体结构。FTIR 研究证实了纳米粒子表面与没食子酸的共轭。GAunps 的抗病毒功效在 Vero 细胞中针对 HSV 感染进行了研究。GAunps 以剂量依赖的方式有效，HSV-1 的 EC 为 32.3μM，HSV-2 的 EC 为 38.6μM。进一步的研究表明，GAunps 可防止病毒附着和侵入 Vero 细胞。在感染细胞中，纳米粒子暴露时间的不同会导致抑制百分比的变化。纳米粒子在 Vero 细胞中的细胞毒性 (CC 972.4μM) 明显低于阿昔洛韦 (CC 561.7μM)，表明其安全性。生物安全金纳米粒子被提议作为病毒化学疗法的更安全替代物。
