Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Ankara, Turkey.
Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Ankara, Turkey.
Mater Sci Eng C Mater Biol Appl. 2018 Aug 1;89:8-14. doi: 10.1016/j.msec.2018.03.021. Epub 2018 Mar 22.
The objective of this study is to develop chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications. With the freeze-drying technique, strontium (Sr) modified C/MMT composite scaffold with an interconnected porous structure was produced. X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold. One of the aims of this study was to document the release of Sr from the non-modified and modified scaffolds into the cell culture medium. The biocompatibility of composite scaffolds was evaluated in cell cultures. Human osteoblasts (hOBs) were cultured, expanded and seeded on Sr-modified and non-modified C/MMT scaffolds. In-vitro cell viability and proliferation were investigated using MTT (3‑(4,5‑dimethylthiasol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and DNA content analysis. Live/dead cell staining assay and SEM were used for evaluating the cell-laden constructs. In-vitro studies showed that C/MMT scaffolds had no negative effects on osteoblasts. Ions present in the MMT were released into the cell culture medium, to induce osteoblast activity in the C/MMT scaffold system. Findings indicate that Sr modification of MMT-chitosan improves scaffold properties, suggesting Sr-modified C/MMT composite may be a promising biomaterial for bone tissue engineering.
本研究旨在开发基于性能改善的壳聚糖/蒙脱土（C/MMT）复合支架，以应用于骨组织工程。采用冷冻干燥技术，制备了具有互穿多孔结构的锶（Sr）改性 C/MMT 复合支架。采用 X 射线衍射、傅里叶变换红外光谱、热重分析和扫描电子显微镜（SEM）研究了复合支架的结构性能、表面形貌和孔隙率。本研究的目的之一是记录 Sr 从未改性和改性支架中释放到细胞培养基中的情况。通过细胞培养评估了复合支架的生物相容性。培养、扩增和接种人成骨细胞（hOBs）到 Sr 改性和未改性的 C/MMT 支架上。通过 MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）测定法和 DNA 含量分析，研究了细胞活力和增殖情况。采用活/死细胞染色和 SEM 评估细胞负载构建体。体外研究表明，C/MMT 支架对成骨细胞没有负面影响。MMT 中存在的离子释放到细胞培养基中，从而在 C/MMT 支架系统中诱导成骨细胞活性。研究结果表明，MMT-壳聚糖的 Sr 改性改善了支架性能，表明 Sr 改性 C/MMT 复合材料可能是一种有前途的骨组织工程生物材料。
