Barik Debyashreeta, Kundu Koustav, Dash Mamoni
Institute of Life Sciences Nalco Square Odisha India
School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT) University Bhubaneswar Odisha 751024 India.
RSC Adv. 2021 Sep 10;11(48):30329-30342. doi: 10.1039/d1ra04803a. eCollection 2021 Sep 6.
The role of polymers has played a crucial role in developing templates that can promote regeneration as tissue-engineered matrices. The present study aims to develop functional matrices involving the protein mucin. The mucin used in this study is characterised using MALDI-TOF TOF and CD spectroscopy prior to conjugation. Thereupon, a hybrid scaffold comprising of a polysaccharide, chitosan, chemically conjugated to a protein, mucin, and encapsulated with montmorillonite is developed. Grafting of hydroxyethyl methacrylate (HEMA) is done to overcome the issue of mechanical weakness that mucin hydrogels usually undergo. It was observed that the presence of montmorillonite led to the stability of the hydrogels. The conjugations with varied ratios of the polysaccharide and protein were characterized using spectroscopic techniques. The prepared gels showed appreciable material properties in terms of water uptake and porosity. Hydrogels with different ratios of the polysaccharide and protein were evaluated for their biocompatibility. The biological evaluation of the hydrogels was performed with MC3T3E1 and C2C12 cell lines indicating their potential for wider tissue engineering applications.
聚合物在开发可作为组织工程基质促进再生的模板方面发挥了关键作用。本研究旨在开发包含蛋白质粘蛋白的功能性基质。本研究中使用的粘蛋白在共轭之前通过基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF TOF）和圆二色光谱（CD）进行表征。随后，开发了一种混合支架，该支架由与蛋白质粘蛋白化学共轭的多糖壳聚糖组成，并用蒙脱石包裹。进行甲基丙烯酸羟乙酯（HEMA）接枝以克服粘蛋白水凝胶通常存在的机械强度不足问题。观察到蒙脱石的存在导致水凝胶的稳定性。使用光谱技术对多糖和蛋白质不同比例的共轭物进行表征。制备的凝胶在吸水和孔隙率方面表现出可观的材料性能。评估了不同比例多糖和蛋白质的水凝胶的生物相容性。使用MC3T3E1和C2C12细胞系对水凝胶进行生物学评估，表明它们在更广泛的组织工程应用中的潜力。
