Department of Bionanotechnology, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do 461-701, Republic of Korea.
Department of Bionanotechnology, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do 461-701, Republic of Korea.
Mater Sci Eng C Mater Biol Appl. 2018 Aug 1;89:87-94. doi: 10.1016/j.msec.2018.03.020. Epub 2018 Mar 27.
In this study, we synthesized a novel metal flavonoid complex and investigated its effects on the non-small cell lung cancer cell lines, A549 and toxicity on the human dermal fibroblast cell lines, HDFa. H, C NMR, single crystal X-ray diffraction and elemental micro analysis (C,H,N,S/O) were used to characterize the synthesized kaempferol-based Ru (II) complex. Cell toxicity was studied using MTT assay and electric cell substrate impedance sensing (ECIS). It was evident from the MTT results that no significant cytotoxicity in HDFa cells occurs with the synthesized complex, but in case of A549 cells, significant cytotoxicity was observed even at low concentrations (10-20 μm). In addition, the effect of the newly synthesized complex on the A549 cell line was studied by investigating the cellular damage via atomic force microscopy and DNA fragmentation assay. The obtained results revealed that the synthesized complex was able to inhibit the cancer cells and have shown moderate anticancer activity against A549 cancer cell lines. In addition, it was evident that the complex was more active than kaempferol and well tolerated by normal cell lines.
在这项研究中，我们合成了一种新型的金属黄酮类配合物，并研究了它对非小细胞肺癌细胞系 A549 的影响及其对人皮肤成纤维细胞系 HDFa 的毒性。采用 H、C NMR、单晶 X 射线衍射和元素微量分析（C、H、N、S/O）对合成的基于山奈酚的 Ru（II）配合物进行了表征。采用 MTT 法和电化学细胞基质阻抗感应（ECIS）研究细胞毒性。MTT 结果表明，合成的配合物在 HDFa 细胞中没有明显的细胞毒性，但在 A549 细胞中，即使在低浓度（10-20μm）下也观察到明显的细胞毒性。此外，还通过原子力显微镜和 DNA 片段化分析研究了新合成的配合物对 A549 细胞系的影响。结果表明，合成的配合物能够抑制癌细胞，对 A549 癌细胞系表现出中等的抗癌活性。此外，该配合物比山奈酚更活跃，对正常细胞系的耐受性良好。
