Struk Sylwia, Braem Lukas, Walton Alan, De Keyser Annick, Boyer François-Didier, Persiau Geert, De Jaeger Geert, Gevaert Kris, Goormachtig Sofie
Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Center for Plant Systems Biology, VIB, Ghent, Belgium.
Front Plant Sci. 2018 Apr 26;9:528. doi: 10.3389/fpls.2018.00528. eCollection 2018.
Phytohormones tightly regulate plant growth by integrating changing environmental and developmental cues. Although the key players have been identified in many plant hormonal pathways, the molecular mechanisms and mode of action of perception and signaling remain incompletely resolved. Characterization of protein partners of known signaling components provides insight into the formed protein complexes, but, unless quantification is involved, does not deliver much, if any, information about the dynamics of the induced or disrupted protein complexes. Therefore, in proteomics research, the discovery of what actually triggers, regulates or interrupts the composition of protein complexes is gaining importance. Here, tandem affinity purification coupled to mass spectrometry (TAP-MS) is combined with label-free quantification (LFQ) to a highly valuable tool to detect physiologically relevant, dynamic protein-protein interactions in cell cultures. To demonstrate its potential, we focus on the signaling pathway of one of the most recently discovered phytohormones, strigolactones.
植物激素通过整合不断变化的环境和发育线索来严格调控植物生长。尽管许多植物激素途径中的关键参与者已被确定，但感知和信号传导的分子机制及作用方式仍未完全阐明。已知信号成分的蛋白质伙伴的表征有助于深入了解所形成的蛋白质复合物，但是，除非涉及定量分析，否则几乎无法提供有关诱导或破坏的蛋白质复合物动态变化的任何信息。因此，在蛋白质组学研究中，发现究竟是什么触发、调节或中断蛋白质复合物的组成变得越来越重要。在这里，串联亲和纯化与质谱联用（TAP-MS）与无标记定量（LFQ）相结合，成为一种在细胞培养中检测生理相关的动态蛋白质-蛋白质相互作用的极有价值的工具。为了证明其潜力，我们聚焦于最近发现的一种植物激素独脚金内酯的信号通路。
