State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
Analyst. 2018 May 29;143(11):2641-2647. doi: 10.1039/c8an00586a.
Reactive oxygen species (ROS), especially HOCl/ClO-, have been demonstrated to play essential roles in both physiological and pathological processes, and an abnormal level of HOCl/ClO- is related to some diseases. In this work, a very fast responsive (within 30 seconds) porphyrin-based fluorescent probe, TPP-TCF, for ClO- with a NIR emissive wavelength was prepared. This probe exhibited excellent selectivity towards ClO- and would not be interfered with by other ROS and typical nucleophiles. The limit of detection (LOD) for ClO- was evaluated to be 0.29 μM, indicating high sensitivity towards ClO-. In further bioimaging experiments, TPP-TCF displayed low-cytotoxicity and good cell penetrability for recognizing exogenous ClO- in HeLa cells. Moreover, this probe was successfully applied in imaging endogenous ClO- in living animals.
活性氧（ROS），尤其是次氯酸（HOCl/ClO-），已被证明在生理和病理过程中发挥着重要作用，而 HOCl/ClO- 的异常水平与一些疾病有关。在这项工作中，我们制备了一种基于卟啉的荧光探针 TPP-TCF，它对具有近红外发射波长的 ClO- 具有非常快速的响应（30 秒内）。该探针对 ClO-具有优异的选择性，不会受到其他 ROS 和典型亲核试剂的干扰。ClO-的检测限（LOD）评估为 0.29 μM，表明对 ClO-具有高灵敏度。在进一步的生物成像实验中，TPP-TCF 显示出低细胞毒性和良好的细胞通透性，可用于识别 HeLa 细胞中的外源性 ClO-。此外，该探针成功应用于活体动物内源性 ClO-的成像。
