一种新型的近红外发射探针，具有较大的斯托克斯位移，可用于活细胞中次氯酸盐的检测和成像。
A novel NIR-emissive probe with large Stokes shift for hypochlorite detection and imaging in living cells.
机构信息
Center of Basic Molecular Science, Department of chemistry, Tsinghua University, Beijing 100084, China.
Center of Basic Molecular Science, Department of chemistry, Tsinghua University, Beijing 100084, China.
出版信息
Talanta. 2019 May 1;196:352-356. doi: 10.1016/j.talanta.2018.12.086. Epub 2018 Dec 26.
Due to the importance of hypochlorite (ClO) in the process of life, a near-infrared region (NIR) emissive probe (DCPO-DMTC) with large Stokes shift was synthesized for the selective detection of hypochlorite. This probe detects exogenous and endogenous hypochlorite via "oxidative deprotection" of dimethylthiocarbamate-protected phenolic hydroxyl groups. The response was monitored by time-course UV-Vis and fluorescent spectroscopy. The emission response of the probe to ClO presented a good linear relationship in the 0-100 µM concentration range, and the LOD of this probe was 164 nM. The probe was used to successfully visualize endogenous ClO generation in RAW 246.7 cells under external stimulation.
由于次氯酸盐 (ClO) 在生命过程中的重要性，合成了一种具有大斯托克斯位移的近红外区域 (NIR) 发射探针 (DCPO-DMTC)，用于选择性检测次氯酸盐。该探针通过保护的酚羟基的“氧化去保护”来检测外源性和内源性次氯酸盐。通过时间分辨的紫外-可见和荧光光谱监测响应。探针对 ClO 的发射响应在 0-100µM 浓度范围内呈现出良好的线性关系，该探针的 LOD 为 164nM。该探针成功地用于在外部刺激下可视化 RAW 246.7 细胞内内源性 ClO 的产生。
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