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一种用于在活肿瘤细胞中检测和成像人NAD(P)H:醌氧化还原酶1（hNQO1）的高选择性荧光探针。

A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells.

作者信息

Zhu Ya, Han Jialing, Zhang Qian, Zhao Zhou, Wang Jin, Xu Xiaowei, Hao Haiping, Zhang Jun

机构信息

School of Pharmacy, China Pharmaceutical University 210009 Nanjing China

School of Pharmacy, Nanjing Medical University 211166 Nanjing China

出版信息

RSC Adv. 2019 Aug 27;9(46):26729-26733. doi: 10.1039/c9ra05650e. eCollection 2019 Aug 23.

DOI:10.1039/c9ra05650e
PMID:35528556
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9070553/
Abstract

Human NAD(P)H:quinone oxidoreductase (hNQO1) can be used as a biomarker for the early diagnosis of cancer. In this article, a novel fluorescent probe 1 for detecting hNQO1 was designed and synthesized by using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) derivative (TCF-OH) as a fluorophore and quinone propionic acid (QPA) as a recognition group. The probe, which has high selectivity for hNQO1 and a Stokes shift of about 117 nm, shows a linear relationship with hNQO1 concentrations in the range of 0.25-3 μg ml with the color changes from yellow to red and was successfully applied to intracellular hNQO1 imaging.

摘要

人源烟酰胺腺嘌呤二核苷酸磷酸（NAD(P)H）：醌氧化还原酶（hNQO1）可作为癌症早期诊断的生物标志物。在本文中，一种用于检测hNQO1的新型荧光探针1被设计并合成，该探针以2-二氰基亚甲基-3-氰基-4,5,5-三甲基-2,5-二氢呋喃（TCF）衍生物（TCF-OH）作为荧光团，以醌丙酸（QPA）作为识别基团。该探针对hNQO1具有高选择性，斯托克斯位移约为117 nm，在0.25 - 3 μg/ml范围内与hNQO1浓度呈线性关系，颜色从黄色变为红色，并成功应用于细胞内hNQO1成像。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97c/9070553/3654f97b1b12/c9ra05650e-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97c/9070553/5fa84bae2832/c9ra05650e-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97c/9070553/64da797ab003/c9ra05650e-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97c/9070553/274b2a564d7c/c9ra05650e-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97c/9070553/5d44c65a3e37/c9ra05650e-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97c/9070553/3654f97b1b12/c9ra05650e-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97c/9070553/5fa84bae2832/c9ra05650e-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97c/9070553/64da797ab003/c9ra05650e-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97c/9070553/274b2a564d7c/c9ra05650e-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97c/9070553/5d44c65a3e37/c9ra05650e-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97c/9070553/3654f97b1b12/c9ra05650e-f4.jpg

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