Larche M, Lamb J R, O'Hehir R E, Imami-Shita N, Zanders E D, Quint D E, Moqbel R, Ritter M A
Department of Immunology, Royal Postgraduate Medical School, London, UK.
Immunology. 1988 Dec;65(4):617-22.
The complex pleiotropic effects of the T-cell derived lymphokine interleukin-4 (IL-4) are becoming increasingly well documented; however, functional studies have been hampered by the lack of reagents directed against the receptor for this factor. In this report, we present data which suggest that the monoclonal antibody MR6 binds to the human interleukin-4 receptor (IL-4R). Addition of MR6 to cultures of T cells proliferating in response to IL-4 inhibited this response in a dose-dependent fashion, giving total inhibition at 10 micrograms/ml. Similarly, the IL-4-dependent production of specific antigen-induced IgE by B-cell populations was completely abrogated by MR6. Flow cytometric studies of the modulation of cell surface molecules after T-cell activation suggest that expression of the molecule detected by MR6 (p145-MR6) correlates inversely with that of the interleukin-2 receptor (IL-2R). These data, together with the previously determined molecular weight and tissue distribution of this molecule, strongly indicate that MR6 binds to the human IL-4R.
T细胞衍生的淋巴因子白细胞介素-4（IL-4）具有复杂的多效性作用，这一点已得到越来越充分的记录；然而，由于缺乏针对该因子受体的试剂，功能研究受到了阻碍。在本报告中，我们提供的数据表明单克隆抗体MR6可与人白细胞介素-4受体（IL-4R）结合。将MR6添加到因IL-4而增殖的T细胞培养物中，会以剂量依赖的方式抑制这种反应，在10微克/毫升时可实现完全抑制。同样，B细胞群体依赖IL-4产生的特异性抗原诱导的IgE也被MR6完全消除。对T细胞活化后细胞表面分子调节的流式细胞术研究表明，MR6检测到的分子（p145-MR6）的表达与白细胞介素-2受体（IL-2R）的表达呈负相关。这些数据，连同先前确定的该分子的分子量和组织分布，有力地表明MR6与人IL-4R结合。
