Kämpfer Peter, Glaeser Stefanie P, Gräber Marco, Rabenstein Andreas, Kuever Jan
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Department of Microbiology, Bremen Institute for Materials Testing, Bremen, Germany.
Int J Syst Evol Microbiol. 2018 Jul;68(7):2240-2241. doi: 10.1099/ijsem.0.002817. Epub 2018 May 14.
Qingshengfania soli DSM 103870 was compared with Pseudochelatococcus lubricantis MPA 1113 to clarify the taxonomic relationship of both species because of their high phylogenetic relationship. 16S rRNA gene sequence comparisons demonstrated that these species share 100 % sequence similarity. Investigation of fatty acid patterns, substrate utilization, and matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) profiles displayed no striking differences between the type strains of both species. DNA-DNA hybridization between both strains showed a 95 % (reciprocal 82 %) similarity, which clearly demonstrated that both strains are members of the same species. Due to priority of publication and validation of the name, Qingshengfania soli is reclassified as Pseudochelatococcus lubricantis, based on the estimated phylogenetic position derived from 16S rRNA gene sequence data, fatty acid, biochemical data, MALDI-TOF, and DNA-DNA hybridization results.
由于青生土壤球菌DSM 103870与润滑假螯球菌MPA 1113具有较高的系统发育关系,为明确这两个物种的分类关系,对它们进行了比较。16S rRNA基因序列比较表明,这些物种具有100%的序列相似性。对脂肪酸模式、底物利用和基质辅助激光解吸/电离飞行时间(MALDI-TOF)图谱的研究表明,这两个物种的模式菌株之间没有显著差异。两株菌之间的DNA-DNA杂交显示相似性为95%(相互为82%),这清楚地表明这两株菌是同一物种的成员。基于从16S rRNA基因序列数据、脂肪酸、生化数据、MALDI-TOF和DNA-DNA杂交结果推断的系统发育位置,由于发表和名称验证的优先权,青生土壤球菌被重新分类为润滑假螯球菌。