Amorim da Silva Renato, Renovato Raissa Santana, Silva Hannah Tsuruzaki Kirzner de Barros E, Marques Maria Luiza Didier, Oliveira Pollyanne Raysa Fernandes, Carvalho-Reis Jéssica de Crasto Souza, Bartley Paul M, Katzer Frank, Samico-Fernandes Érika Fernanda Torres, Melo Renata Pimentel Bandeira de, Mota Rinaldo Aparecido
Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, Brazil.
Moredun Research Institute, Pentlands Science Park, Penicuik, EH26 0PZ, Scotland, United Kingdom.
Curr Res Parasitol Vector Borne Dis. 2024 Aug 14;6:100207. doi: 10.1016/j.crpvbd.2024.100207. eCollection 2024.
This study aimed to evaluate the presence and viability of in chickens intended for human consumption in the Pernambuco State, Brazil. Blood and tissue samples were collected from 25 chickens sold in markets in Recife, Pernambuco. Samples were evaluated by indirect immunofluorescence assay (IFA) to detect antibodies to . Pools of brain and heart of seropositive chickens were subjected to bioassay in two Swiss Webster mice, which were evaluated for 45 days then tested by IFA to detect seroconversion. The mice were euthanized, and their brains were evaluated for cysts. Peritoneal lavage was also conducted in mice that exhibited clinical signs. Brains containing cysts or peritoneal lavage with tachyzoites were inoculated into MA-104 cells. Brains of mice inoculated with the same tissue were pooled and analysed by ITS1-PCR. We obtained a frequency of antibodies to of 68.00% (17/25) in chickens, and a seroconversion rate of 70.58% (24/34) in mice. Detection of ITS1 DNA confirmed an isolation rate of 41.1% (7/17). Three isolates were characterized by mnPCR-RFLP as genotypes ToxoDB#36 and ToxoDB#114. We highlight the occurrence of ToxoDB#36 in chickens in Pernambuco State and the parasites' viability in chickens intended for human consumption.
本研究旨在评估巴西伯南布哥州供人类食用的鸡中[具体病原体名称缺失]的存在情况和生存能力。从伯南布哥州累西腓市场上出售的25只鸡采集血液和组织样本。通过间接免疫荧光试验（IFA）评估样本，以检测针对[具体病原体名称缺失]的抗体。将血清阳性鸡的脑和心脏混合样本在两只瑞士韦伯斯特小鼠中进行生物测定，对小鼠评估45天，然后通过IFA检测血清转化情况。对小鼠实施安乐死，并评估其脑内的囊肿。对出现临床症状的小鼠也进行腹腔灌洗。将含有囊肿的脑或含有速殖子的腹腔灌洗物接种到MA - 104细胞中。将接种相同组织的小鼠脑混合，并通过ITS1 - PCR进行分析。我们在鸡中获得针对[具体病原体名称缺失]的抗体频率为68.00%（17/25），在小鼠中的血清转化率为70.58%（24/34）。检测[具体病原体名称缺失]的ITS1 DNA证实分离率为41.1%（7/17）。通过多重巢式PCR - 限制性片段长度多态性分析鉴定出三个分离株为ToxoDB#36和ToxoDB#114基因型。我们强调了伯南布哥州鸡中ToxoDB#36的出现情况以及该寄生虫在供人类食用的鸡中的生存能力。
