用于皮肤绷带的电响应氧化石墨烯水凝胶：明胶和胰蛋白酶固定化的结果。
Electro-responsive graphene oxide hydrogels for skin bandages: The outcome of gelatin and trypsin immobilization.
机构信息
Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Center for Musculoskeletal Surgery, Charitéplatz 1, 10117 Berlin, Germany; Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany.
Children's Cancer Institute, Lowy Cancer Research Centre, University of New South Wales, Sydney, Australia; Australian Centre for NanoMedicine, ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales, NSW, Sydney, Australia.
出版信息
Int J Pharm. 2018 Jul 30;546(1-2):50-60. doi: 10.1016/j.ijpharm.2018.05.027. Epub 2018 May 11.
A free radical polymerization method was adopted for the fabrication of hybrid hydrogel films based on acrylamide and polyethylene glycol dimethacrylate as plasticizing and crosslinking agents, respectively, to be employed as smart skin bandages. Electro-sensitivity, biocompatibility and proteolytic properties were conferred to the final polymer networks by introducing graphene oxide (0.5% w/w), gelatin or trypsin (10% w/w) in the polymerization feed. The physical chemical and mechanical characterization of hybrid materials was performed by means of determination of protein content, Raman spectroscopy, thermogravimetric analysis and measurement of tensile strength. The evaluation of both water affinity and curcumin release profiles (analyzed by suitable mathematical modelling) upon application of an external electric stimulation in the 0-48 voltage range, confirmed the possibility to modulate the release kinetics. Proper proteolytic tests showed that the trypsin enzymatic activity was retained by 80% upon immobilization. Moreover, for all samples, we observed a viability higher than 94% in normal human fibroblast cells (MRC-5), while a reduction of methicillin-resistant Staphylococcus aureus CFU mL (90%) was obtained with curcumin loaded samples.
采用自由基聚合的方法制备了基于丙烯酰胺和聚乙二醇二甲基丙烯酸酯的混合水凝胶薄膜，分别作为增塑剂和交联剂，用作智能皮肤绷带。通过在聚合进料中引入氧化石墨烯（0.5%w/w）、明胶或胰蛋白酶（10%w/w），赋予最终聚合物网络电敏感性、生物相容性和蛋白水解特性。通过测定蛋白质含量、拉曼光谱、热重分析和拉伸强度来对混合材料的物理化学和机械性能进行表征。通过在 0-48 伏电压范围内施加外部电刺激来评估水亲和力和姜黄素释放曲线（通过适当的数学模型进行分析），证实了可以调节释放动力学。适当的蛋白水解测试表明，在固定化过程中，胰蛋白酶的酶活性保留了 80%。此外，对于所有样品，我们在正常的人成纤维细胞（MRC-5）中观察到高于 94%的活力，而负载姜黄素的样品则获得了耐甲氧西林金黄色葡萄球菌 CFU mL（90%）的减少。
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