Kelly Aoife, Grabiec Aleksander M, Travis Mark A
Manchester Collaborative Centre for Inflammation Research, University of Manchester, Manchester, UK.
Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, UK.
Methods Mol Biol. 2018;1784:1-11. doi: 10.1007/978-1-4939-7837-3_1.
The study of human macrophages is often hampered by access to tissue and inability of this cell type to survive in vitro following isolation. The culture of human monocyte-derived macrophages (MDMs) represents a tool to study macrophages, with monocytes known to give rise to tissue macrophages influenced by certain environmental cues. Here we describe a method of culturing monocyte-derived macrophages from CD14+ blood monocytes and polarization toward different macrophage phenotypes.
对人类巨噬细胞的研究常常受到获取组织的限制，以及这种细胞类型在分离后无法在体外存活的影响。人类单核细胞衍生巨噬细胞（MDM）的培养是研究巨噬细胞的一种工具，已知单核细胞会在某些环境线索的影响下分化为组织巨噬细胞。在这里，我们描述了一种从CD14 +血液单核细胞培养单核细胞衍生巨噬细胞并使其极化成为不同巨噬细胞表型的方法。
