Assouvie Anaïs, Daley-Bauer Lisa P, Rousselet Germain
CEA/DRF/Jacob/iRCM/LRTS, INSERM U967, Fontenay aux Roses, France.
Université Paris-Diderot, Paris, France.
Methods Mol Biol. 2018;1784:29-33. doi: 10.1007/978-1-4939-7837-3_3.
Bone marrow-derived macrophages (BMDM) are primary macrophages obtained by in vitro differentiation of bone marrow cells in the presence of macrophage colony-stimulating factor (M-CSF or CSF1). They are easy to obtain in high yields, can be stored by freezing, and can be obtained from genetically modified mice strains. They are therefore widely used as prototypical macrophages for in vitro studies. In this chapter, we present the method for obtaining BMDMs and freezing them.
骨髓来源的巨噬细胞（BMDM）是在巨噬细胞集落刺激因子（M-CSF或CSF1）存在下通过骨髓细胞体外分化获得的原代巨噬细胞。它们易于高产获得，可通过冷冻保存，并且可以从基因改造的小鼠品系中获得。因此，它们被广泛用作体外研究的典型巨噬细胞。在本章中，我们介绍获得BMDM并将其冷冻的方法。
