Rios Francisco J, Touyz Rhian M, Montezano Augusto C
Institute of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.
Kidney Research Centre, Department of Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.
Methods Mol Biol. 2017;1527:297-309. doi: 10.1007/978-1-4939-6625-7_23.
Macrophages play a major role in inflammation, wound healing, and tissue repair. Infiltrated monocytes differentiate into different macrophage subtypes with protective or pathogenic activities in vascular lesions. In the heart and vascular tissues, pathological activation promotes cardiovascular inflammation and remodeling and there is increasing evidence that macrophages play important mechanisms in this environment. Primary murine macrophages can be obtained from: bone marrow by different treatments (granulocyte-macrophage colony-stimulating factor-GM-CSF, macrophage colony-stimulating factor-M-CSF or supernatant of murine fibroblast L929), peritoneal cavity (resident or thioglycolate elicit macrophages), from the lung (alveolar macrophages) or from adipose tissue. In this chapter we describe some protocols to obtain primary murine macrophages and how to identify a pure macrophage population or activation phenotypes using different markers.
巨噬细胞在炎症、伤口愈合和组织修复中起主要作用。浸润的单核细胞在血管病变中分化为具有保护或致病活性的不同巨噬细胞亚型。在心脏和血管组织中，病理激活会促进心血管炎症和重塑，并且越来越多的证据表明巨噬细胞在这种环境中发挥重要作用。原代小鼠巨噬细胞可从以下来源获得：通过不同处理的骨髓（粒细胞-巨噬细胞集落刺激因子-GM-CSF、巨噬细胞集落刺激因子-M-CSF或小鼠成纤维细胞L929的上清液）、腹腔（驻留或巯基乙酸诱导的巨噬细胞）、肺（肺泡巨噬细胞）或脂肪组织。在本章中，我们描述了一些获取原代小鼠巨噬细胞的方案，以及如何使用不同标记物鉴定纯巨噬细胞群体或激活表型。
