Taylor Lewis, Recio Carlota, Greaves David R, Iqbal Asif J
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.
Methods Mol Biol. 2018;1784:197-214. doi: 10.1007/978-1-4939-7837-3_19.
The timely recruitment of innate and adaptive immune cells to sites of inflammation and repair is essential for host defense against pathogens and repair of damaged tissues. The development of bioassays such as in vitro chemotaxis assays played an important role in the original purification of chemoattractant cytokines including interleukin-1 and the CC and CXC chemokines. The earliest chemotaxis methods were based on the principle of the Boyden chamber, first described in 1962. In this chapter we give detailed protocols for more recent techniques that allow determination of macrophage chemotaxis in real time. These techniques have given new insights into the regulation of macrophage responses to chemotaxis in vitro and in vivo.
先天免疫细胞和适应性免疫细胞及时募集到炎症和修复部位，对于宿主抵御病原体和修复受损组织至关重要。诸如体外趋化性分析等生物分析方法的发展，在最初纯化包括白细胞介素-1以及CC和CXC趋化因子在内的趋化因子细胞因子方面发挥了重要作用。最早的趋化性方法基于1962年首次描述的博伊登小室原理。在本章中，我们给出了更近期技术的详细方案，这些技术可实时测定巨噬细胞趋化性。这些技术为体外和体内巨噬细胞对趋化性反应的调控提供了新的见解。
