Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou, China.
Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.
J Nat Med. 2018 Sep;72(4):846-856. doi: 10.1007/s11418-018-1215-9. Epub 2018 May 14.
Symptoms of disseminated intravascular coagulation (DIC) include thromboembolism, acute attrition bleeding and multiple organ failure. Genistein isolated from leguminous plants has been shown to be effective in oxidation resistance and tumor inhibition. The present study was designed to evaluate the therapeutic effects of genistein in DIC and preliminarily discuss the mechanisms regarding the anti-inflammatory and anticoagulant effect of genistein. Swiss mice were randomly divided into the following groups-(1) lipopolysaccharide (LPS), (2) genistein, (3) dimethyl sulfoxide (DMSO, the non-major solvent component of genistein), (4) DMSO + LPS, (5) saline control group, and (6) heparin control group. LPS was injected intraperitoneally in all the groups except the DMSO group and saline control group. Our results significantly showed that the morphological structure of the liver and kidneys was improved and the fiber protein deposition was decreased, with remarkable improvement of coagulation indicators, function indicators and inflammatory factors in the genistein treatment group compared with the LPS group. In vitro phosphorylated-nuclear factor kappa-light-chain-enhancer of activated B cells and interleukin-6 were obviously reduced in the genistein treatment group compared with the LPS group in RAW 264.7 murine macrophage cells. All the results suggested that genistein has the function of alleviating and treating LPS-induced DIC by anti-inflammatory and anticoagulation effects. We tentatively propose that genistein is a potential drug for auxiliary treatment of DIC.
弥散性血管内凝血（DIC）的症状包括血栓栓塞、急性消耗性出血和多器官衰竭。从豆科植物中分离出的染料木黄酮已被证明具有抗氧化和抑制肿瘤的作用。本研究旨在评估染料木黄酮在 DIC 中的治疗效果，并初步探讨其抗炎和抗凝作用的机制。将瑞士小鼠随机分为以下几组：（1）脂多糖（LPS）组、（2）染料木黄酮组、（3）二甲基亚砜（DMSO，染料木黄酮的非主要溶剂成分）组、（4）DMSO+LPS 组、（5）生理盐水对照组和（6）肝素对照组。除 DMSO 组和生理盐水对照组外，所有组均腹腔内注射 LPS。我们的结果表明，与 LPS 组相比，染料木黄酮治疗组的肝、肾形态结构得到改善，纤维蛋白沉积减少，凝血指标、功能指标和炎症因子明显改善。在 RAW 264.7 小鼠巨噬细胞中，与 LPS 组相比，染料木黄酮治疗组的磷酸化核因子 kappaB 轻链增强子的 B 细胞和白细胞介素-6 明显减少。所有结果表明，染料木黄酮通过抗炎和抗凝作用具有缓解和治疗 LPS 诱导的 DIC 的功能。我们推测染料木黄酮是 DIC 辅助治疗的一种潜在药物。
