Department of Gynecology, Maternal and Child Health Care Hospital, Laiwu City, Shandong Province, China.
Eur Rev Med Pharmacol Sci. 2018 Apr;22(8):2207-2211. doi: 10.26355/eurrev_201804_14805.
This study explores the possible relation between cervical infections with Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH) and the occurrence of recurrent spontaneous abortion (RSA).
132 patients with RSA (observation group) and 96 normal pregnancy volunteers undergoing planned abortions (control group) were selected successively and enrolled in the investigation. Cervical secretion samples were obtained for each subject. Bacterial cultures were started to detect UU, MH and other bacterial infections, and fluorescence quantitative PCR was used to detect gene copy number in chorion and decidual tissues. Additionally, ELISA (enzyme-linked immunosorbent assay) was used to detect anticardiolipin antibody (ACA) to rate positivity of Immunoglobulin M (IgM) and IgG in secretions, and Western blot was applied to quantify the expression levels of Interleukin (IL)-6, tumor necrosis factor-α (TNF-α), prostacyclin (PGI2) and bax/bcl-2.
Our results showed the UU, MH, and overall bacterial infection rate of chorionic and decidual tissues, and the gene copy number of UU, MH were higher in the observation group than those in the control group (p<0.05). Moreover, the ACA-IgM and IgG positive rates in secretions of the observation group were significantly higher than those in the control group (p<0.05). Finally, the expression levels of IL-6, TNF-α, PGI2, and bax/bcl-2 were higher than those in the control group as well (p<0.05).
Our results support the notion that RSA might be associated with UU and MH infection, could influence the occurrence of other bacterial infections and could stimulate ACA expression, inflammatory response, thrombogenesis, and factors associated with cell apoptosis, increasing the risk for an abortion during pregnancy.
本研究探讨解脲脲原体（UU）和人型支原体（MH）感染与复发性自然流产（RSA）之间的可能关系。
连续选取 132 例 RSA 患者（观察组）和 96 例计划流产的正常妊娠志愿者（对照组），采集宫颈分泌物，进行细菌培养，检测 UU、MH 等细菌感染，并采用荧光定量 PCR 检测绒毛膜和蜕膜组织的基因拷贝数。同时采用酶联免疫吸附试验（ELISA）检测分泌物中抗心磷脂抗体（ACA），以评估免疫球蛋白 M（IgM）和 IgG 的阳性率，并用 Western blot 定量分析白细胞介素（IL）-6、肿瘤坏死因子-α（TNF-α）、前列环素（PGI2）和 bax/bcl-2 的表达水平。
观察组的 UU、MH 及绒毛膜和蜕膜组织的总细菌感染率、UU、MH 基因拷贝数均高于对照组（p<0.05）。观察组分泌物中 ACA-IgM 和 IgG 的阳性率明显高于对照组（p<0.05）。最后，观察组的 IL-6、TNF-α、PGI2 和 bax/bcl-2 的表达水平也高于对照组（p<0.05）。
我们的研究结果支持 RSA 可能与 UU 和 MH 感染有关的观点，它可能影响其他细菌感染的发生，并刺激 ACA 表达、炎症反应、血栓形成以及与细胞凋亡相关的因素，增加妊娠期间流产的风险。
