Ruan Y, Li Y, Zhang M, Chen X, Liu Z, Wang S, Jiang S
School of Applied Chemistry and Biological Technology, Postdoctoral Innovation Practice Base, Shenzhen Polytechnic, Shenzhen, Guangdong, China.
School of Forest Resources and Conservation, University of Florida, Gainesville, Florida, U.S.A.
J Microsc. 2018 May 15. doi: 10.1111/jmi.12711.
The endeavours to reveal the tracheal system of insects and some arachnids has a long history. The traditional way to observe a tracheal system in an insect body is by utilising the glycerin immersion method. In this study, we developed the lactic acid immersion method, which reveals a more complete tracheal system. By mounting various types of live specimens or body parts directly into lactic acid, multiple intact and complex tracheal systems were clearly visualised. The lactic acid immersion contributed to revealing tracheal systems by penetrating body tissue while reserving enough time for observation before the penetration of the tracheae. Preliminary comparisons were conducted between lactic acid and other mediae, including glycerin. It turned out that lactic acid immersion provides better details and more distinct structures. In our test, the optimal time for observing the tracheal system was 10-25 min after the organism was immersed in lactic acid.
揭示昆虫和一些蛛形纲动物气管系统的努力有着悠久的历史。观察昆虫体内气管系统的传统方法是使用甘油浸泡法。在本研究中，我们开发了乳酸浸泡法，该方法能揭示更完整的气管系统。通过将各种类型的活体标本或身体部位直接放入乳酸中，可以清晰地看到多个完整且复杂的气管系统。乳酸浸泡通过穿透身体组织来帮助揭示气管系统，同时在气管被穿透之前保留了足够的观察时间。我们对乳酸和其他介质（包括甘油）进行了初步比较。结果表明，乳酸浸泡能提供更好的细节和更清晰的结构。在我们的测试中，观察气管系统的最佳时间是生物体浸入乳酸后10 - 25分钟。
