Department of Biological and Health Sciences, University of Araraquara, UNIARA, São Paulo, Brazil.
Institute of Chemistry, University of Campinas, UNICAMP, Campinas-SP, Brazil.
J Appl Microbiol. 2018 Sep;125(3):777-791. doi: 10.1111/jam.13916. Epub 2018 Jun 13.
Obtain varieties of Gluconacetobacter hansenii from original strain ATCC 23729 with greater efficiency to produce bacterial cellulose (BC) membrane with better dry mass yield for application as support of sustained antimicrobials' drug release.
Application of different chemical and physical conditions (pH, temperature and UV light exposure) to obtain different G. hansenii varieties with high capacity to produce BC membranes. Characterization of the G. hansenii variants was performed by scanning electron microscopy (SEM) and optical microscopy of the colony-forming units. BC membrane produced was characterized by SEM, infrared spectroscopy and X-ray diffraction. The BC produced by variants isolated after incubation at 35°C showed elevated dry mass yield and high capacity of retention and sustained release of ceftriaxone antibiotic with the produced BC by original G. hansenii ATCC 23769 strain subjected to incubation at 28°C and with commercial BC.
The application of different chemical and physical conditions constitutes an important method to obtain varieties of micro-organisms with dissimilar metabolism advantageous in relation to the original strain in the BC production.
These results demonstrate the importance of in vivo studies for the application, in medicine, of BC membranes as support for antimicrobial-sustained release for the skin wound treatment.
从原始菌株 ATCC 23729 中获得更多生产细菌纤维素 (BC) 膜的高效变体，以获得更高的干质量产率，从而将其应用于作为持续释放抗菌药物的支持物。
应用不同的化学和物理条件（pH 值、温度和紫外线照射）来获得具有高生产 BC 膜能力的不同汉逊氏醋酸杆菌变体。通过扫描电子显微镜 (SEM) 和菌落形成单位的光学显微镜对 G. hansenii 变体进行了表征。通过 SEM、红外光谱和 X 射线衍射对所生产的 BC 膜进行了表征。在 35°C 孵育条件下分离出的变体所生产的 BC 具有较高的干质量产率，并且对头孢曲松抗生素的保留和持续释放具有较高的容量，而原始 G. hansenii ATCC 23769 菌株在 28°C 孵育条件下和商用 BC 所生产的 BC 则没有这种效果。
应用不同的化学和物理条件是获得具有不同代谢优势的微生物变体的重要方法，与 BC 生产中的原始菌株相比具有优势。
这些结果表明，在体内研究对于将 BC 膜作为支持物应用于皮肤伤口治疗中的抗菌药物持续释放非常重要。
