Mussano Federico, Genova Tullio, Laurenti Marco, Zicola Elisa, Munaron Luca, Rivolo Paola, Mandracci Pietro, Carossa Stefano
Int J Oral Maxillofac Implants. 2018 May/Jun;33(3):571-579. doi: 10.11607/jomi.6479.
This research aimed to assess whether pink-shaded anodized surfaces could enhance the adhesion of soft tissue cells compared with untreated machined titanium surfaces.
Two types of Ti-Al-V titanium samples were prepared: machined titanium (Ti) and anodized titanium (AnoTi). The microstructure was studied by means of a scanning electron microscope. X-ray photoelectron spectroscopy (XPS) was carried out as well. The wetting properties were investigated by the sessile drop technique with water and diiodomethane. To investigate the biologic response in vitro, the epithelial cell line HaCaT and the fibroblastic cell line NHDF were used. Cell adhesion, morphology, and proliferation were evaluated.
The microstructure of the tested surfaces was irregularly smooth for both types of samples with no relevant morphologic differences. The XPS and HR-XPS performed on the AnoTi samples confirmed the presence of Ti, O, and C, along with Ti oxides. Following the optical contact angle measurements, the anodization process induced a slight transition toward the hydrophobic regime. Consequently, the surface free energy values differed significantly between the anodized and the machined samples. Anodized Ti significantly increased the adhesion and proliferation of both epithelial cells and fibroblasts when compared with the pristine Ti controls.
Compared with the clinical standard, anodized surfaces could enhance the adhesion of the two major cell types within the peri-implant soft tissues, which makes pink anodization a promising option for implant dentistry.
本研究旨在评估与未处理的机械加工钛表面相比，粉色阳极氧化表面是否能增强软组织细胞的黏附。
制备了两种类型的Ti-Al-V钛样品：机械加工钛（Ti）和阳极氧化钛（AnoTi）。通过扫描电子显微镜研究微观结构。还进行了X射线光电子能谱（XPS）分析。采用静滴法用水和二碘甲烷研究润湿性。为了研究体外生物学反应，使用了上皮细胞系HaCaT和成纤维细胞系NHDF。评估细胞黏附、形态和增殖情况。
两种类型样品的测试表面微观结构均为不规则光滑，无明显形态学差异。对AnoTi样品进行的XPS和高分辨XPS证实存在Ti、O和C以及钛氧化物。光学接触角测量后发现，阳极氧化过程导致向疏水状态略有转变。因此，阳极氧化样品和机械加工样品的表面自由能值存在显著差异。与原始Ti对照相比，阳极氧化Ti显著增加了上皮细胞和成纤维细胞的黏附和增殖。
与临床标准相比，阳极氧化表面可增强种植体周围软组织中两种主要细胞类型的黏附，这使得粉色阳极氧化成为种植牙科的一个有前景的选择。
