Department of Biotechnology, 10, Institutional Area, Vasant Kunj, TERI School of Advanced Studies, New Delhi 110070, India.
Research and Scientific Studies Unit, College of Nursing & Allied Health Sciences, Jazan University, Jazan 45142, Saudi Arabia.
Microb Pathog. 2018 Aug;121:238-244. doi: 10.1016/j.micpath.2018.05.017. Epub 2018 May 12.
Bacterial pathogens invade and disrupt the host defense system by means of protein sequences structurally similar at global and local level both. The sharing of homologous sequences between the host and the pathogenic bacteria mediates the infection and defines the concept of molecular mimicry. In this study, various computational approaches were employed to elucidate the pathogenicity of Clostridium botulinum ATCC 3502 at genome-wide level. Genome-wide study revealed that the pathogen mimics the host (Homo sapiens) and unraveled the complex pathogenic pathway of causing infection. The comparative 'omics' approaches helped in selective screening of 'molecular mimicry' candidates followed by the qualitative assessment of the virulence potential and functional enrichment. Overall, this study provides a deep insight into the emergence and surveillance of multidrug resistant C. botulinum ATCC 3502 caused infections. This is the very first report identifying C. botulinum ATCC 3502 proteome enriched similarities to the human host proteins and resulted in the identification of 20 potential mimicry candidates, which were further characterized qualitatively by sub-cellular organization prediction and functional annotation. This study will provide a variety of avenues for future studies related to infectious agents, host-pathogen interactions and the evolution of pathogenesis process.
细菌病原体通过在全球和局部水平上都具有结构相似的蛋白质序列来入侵和破坏宿主防御系统。宿主和致病细菌之间同源序列的共享介导了感染，并定义了分子模拟的概念。在这项研究中，采用了各种计算方法来阐明肉毒梭菌 ATCC 3502 在全基因组水平上的致病性。全基因组研究表明，病原体模拟了宿主（智人），并揭示了导致感染的复杂致病途径。比较“组学”方法有助于选择性筛选“分子模拟”候选物，然后对毒力潜力和功能富集进行定性评估。总的来说，这项研究深入了解了多药耐药性肉毒梭菌 ATCC 3502 引起的感染的出现和监测。这是首次发现肉毒梭菌 ATCC 3502 蛋白质组与人类宿主蛋白具有丰富的相似性，并确定了 20 个潜在的模拟候选物，进一步通过亚细胞组织预测和功能注释对其进行了定性特征描述。这项研究将为与传染病原体、宿主-病原体相互作用和发病机制的进化相关的未来研究提供多种途径。
