Department of Pediatrics, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 513 Parnassus Avenue, San Francisco, California 94143, USA.
Glia. 2013 Sep;61(9):1518-32. doi: 10.1002/glia.22538. Epub 2013 Jul 10.
Developmental regulation of gliogenesis in the mammalian CNS is incompletely understood, in part due to a limited repertoire of lineage-specific genes. We used Aldh1l1-GFP as a marker for gliogenic radial glia and later-stage precursors of developing astrocytes and performed gene expression profiling of these cells. We then used this dataset to identify candidate transcription factors that may serve as glial markers or regulators of glial fate. Our analysis generated a database of developmental stage-related markers of Aldh1l1+ cells between murine embryonic day 13.5-18.5. Using these data we identify the bZIP transcription factor Nfe2l1 and demonstrate that it promotes glial fate under direct Sox9 regulatory control. Thus, this dataset represents a resource for identifying novel regulators of glial development.
哺乳动物中枢神经系统（CNS）中的神经胶质发生的发育调控机制尚未完全阐明，部分原因是谱系特异性基因的种类有限。我们使用 Aldh1l1-GFP 作为神经胶质放射状胶质和发育中星形胶质细胞前体的标记物，并对这些细胞进行了基因表达谱分析。然后，我们使用该数据集来鉴定候选转录因子，这些转录因子可能作为神经胶质标记物或神经胶质命运的调节剂。我们的分析生成了一个与 Aldh1l1+细胞发育阶段相关的标记物数据库，这些细胞来自于小鼠胚胎发育第 13.5-18.5 天。使用这些数据，我们鉴定出 bZIP 转录因子 Nfe2l1，并证明它在 Sox9 直接调控下促进神经胶质命运。因此，该数据集代表了一个用于鉴定神经胶质发育新调控因子的资源。
