The clinical significance of silent mutations with respect to ciprofloxacin resistance in MRSA.

BACKGROUND

The aim of this study was to investigate the genotypic differences between different sequence type MRSA isolates, especially focusing on silent 474 mutations and the relationship between such mutations and ciprofloxacin resistance.

METHODS

Seventy-nine MRSA isolates were obtained for antibiotic susceptibility tests and molecular study.

RESULTS

Among these isolates, we found that the MIC, MIC, and minimum inhibitory concentration (MIC) range of ciprofloxacin were much higher for the isolates without the 474 mutation than for isolates with the 474 mutation. A total of 87.5% of the isolates with the 474 mutation were susceptible to ciprofloxacin, but none of the isolates without the 474 mutation were susceptible to ciprofloxacin. For 27 MRSA isolates without rpo474 silent mutation but with 86/126 silent mutation, all of them belonged to SCC III, and had high ciprofloxacin MIC levels. For another 44 MRSA isolates with rpo474 silent mutation but without 86/126 silent mutation, all of them showed low ciprofloxacin MIC levels, all of them belonged to either SCC IV or V. Furthermore, MRSA ciprofloxacin resistance was found to be associated with the mutations S84L/ S80F or S84L, and S85P/ S80Y.

CONCLUSION

Most occurrences of this 474 silent mutation were found in community acquired-MRSA (CA-MRSA) isolates with susceptibility to most antibiotics, especially for ciprofloxacin and vice versa. Thus, this mutation may help to differentiate the different microbiologic characteristics of MRSA clinical isolates.