Zhang Ping, Wang Li M, Zheng Da W, Lin Tai F, Wei Xiao D, Liu Xiao Y, Wang Hui Q
a College of Life Science and Bioengineering, Beijing University of Technology , Beijing , P. R. China.
J Environ Sci Health B. 2018;53(9):561-566. doi: 10.1080/03601234.2018.1473954. Epub 2018 May 16.
A rapid and quantitative method for the determination of N-Benzylademine (N-BA) was established through the application of surface-enhanced Raman spectroscopy (SERS). The Raman peak intensities of N-BA at 1002 cm positively correlated to N-BA concentrations in sprout extracts. The R reached 0.99, and RSDs calculated below 10% at the concentration range of 0.1 ∼5μg mL. The average recoveries were 80.0% ∼ 98.2% for blank samples intentionally contaminated at differing levels of 0.04, 0.4, and 1 μg g. The whole procedure, including sample preparation and SERS detection, did not exceed 30 min for a set of 6 samples. This study indicates that SERS is a promising technique for rapid tracing analysis and on-site testing of N-BA.
通过表面增强拉曼光谱（SERS）建立了一种快速定量测定N-苄基腺嘌呤（N-BA）的方法。N-BA在1002 cm处的拉曼峰强度与豆芽提取物中N-BA的浓度呈正相关。相关系数R达到0.99，在0.1～5μg/mL浓度范围内计算得到的相对标准偏差（RSD）低于10%。对于故意污染水平分别为0.04、0.4和1μg/g的空白样品，平均回收率为80.0%～98.2%。包括样品制备和SERS检测在内的整个过程，一组6个样品不超过30分钟。本研究表明，SERS是一种用于N-BA快速追踪分析和现场检测的有前途的技术。
