在肺炎克雷伯菌中，当mcr - 1基因与失活的mgrB基因共存时，其对黏菌素耐药性无影响。
mcr-1 Gene Has No Effect on Colistin Resistance When It Coexists with Inactivated mgrB Gene in Klebsiella pneumoniae.
作者信息
Zhang Huichuan, Zhao Dongdong, Shi Qiucheng, Quan Jingjing, Li Xi, Yu Yunsong
机构信息
1 Department of Infectious Diseases, College of Medicine, Sir Run Run Shaw Hospital, Zhejiang University , Hangzhou, Zhejiang, China .
2 Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province , Hangzhou, Zhejiang, China .
出版信息
Microb Drug Resist. 2018 Oct;24(8):1117-1120. doi: 10.1089/mdr.2017.0291. Epub 2018 May 16.
The inactivated mgrB gene and the mcr-1 gene are important mechanisms of colistin resistance in Klebsiella pneumoniae and they are threats to the clinical use of colistin. In this study, mcr-1 gene was cloned into K. pneumoniae strains (XH209 and KP10) and their derived strains (XH209 M and KP10 M), which showed high-level resistance to colistin. The acquisition of the mcr-1 gene led to colistin resistance in XH209 and KP10, but the addition of mcr-1 gene did not cause change of colistin minimum inhibitory concentrations in the XH209 M and KP10 M. In addition, the impact of mcr-1 gene on growth rate showed strain specific in K. pneumoniae. In conclusion, the mcr-1 gene does not cause the same level of colistin resistance as the inactivated mgrB gene in K. pneumoniae. The mcr-1 gene has no effect on colisitin resistance when it coexists with inactivated mgrB gene in K. pneumoniae.
失活的mgrB基因和mcr-1基因是肺炎克雷伯菌对黏菌素耐药的重要机制，它们对黏菌素的临床应用构成威胁。在本研究中，mcr-1基因被克隆到肺炎克雷伯菌菌株（XH209和KP10）及其衍生菌株（XH209 M和KP10 M）中，这些菌株对黏菌素表现出高水平耐药。mcr-1基因的获得导致XH209和KP10对黏菌素耐药，但mcr-1基因的添加并未导致XH209 M和KP10 M中黏菌素最低抑菌浓度的改变。此外，mcr-1基因对肺炎克雷伯菌生长速率的影响具有菌株特异性。总之，在肺炎克雷伯菌中，mcr-1基因不会导致与失活的mgrB基因相同水平的黏菌素耐药。当mcr-1基因与失活的mgrB基因在肺炎克雷伯菌中共存时，mcr-1基因对黏菌素耐药没有影响。
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