Gray E D, Abdin Z H, el Kholy A, Mansour M, Miller L C, Zaher S, Kamel R, Regelmann W E
Department of Pediatrics, University of Minnesota Medical School, Minneapolis.
J Rheumatol. 1988 Nov;15(11):1672-6.
Natural killer cell activity and alterations in cytotoxicity after culture with streptococcal blastogen A and phytohemagglutinin (PHA) were examined in patients with inactive rheumatic heart disease (RHD) and control patients. Natural cytotoxic activity of mononuclear cells (MNC) did not differ between RHD and control patients with either peripheral blood or tonsils. In cultured blood MNC the level of cytotoxic activity stimulated by blastogen A was significantly greater in patients with RHD at all effector:target cell ratios. These differences in cytotoxic activity were not observed with cultured tonsillar MNC. In similar experiments with a different group of patients, culture with PHA or blastogen A both produced a significantly greater increase in cytotoxic activity in blood MNC from patients with RHD. The increase was significantly lower with PHA than with blastogen A. The ability of mitogens to differentially augment cytotoxic activity in cells from the blood of patients with RHD implies that a population of cells exists in these patients that could be activated during acute rheumatic fever to play a role in pathogenesis.
在非活动期风湿性心脏病（RHD）患者和对照患者中，检测了用链球菌促分裂原A和植物血凝素（PHA）培养后自然杀伤细胞活性及细胞毒性的变化。无论是外周血还是扁桃体，RHD患者和对照患者的单核细胞（MNC）的自然细胞毒性活性均无差异。在培养的血液MNC中，在所有效应细胞：靶细胞比例下，促分裂原A刺激的细胞毒性活性水平在RHD患者中显著更高。培养的扁桃体MNC未观察到细胞毒性活性的这些差异。在另一组患者的类似实验中，用PHA或促分裂原A培养均使RHD患者血液MNC的细胞毒性活性显著增加。PHA诱导的增加显著低于促分裂原A。有丝分裂原在RHD患者血液细胞中差异增强细胞毒性活性的能力表明，这些患者中存在一群细胞，在急性风湿热期间可能被激活并在发病机制中起作用。
