Harris D, Desai S, Smieja M, Rutherford C, Mertz D, Pernica J M
Department of Medicine, McMaster University, Hamilton, ON.
Department of Pediat rics, McMaster University, Hamilton, ON.
Can Commun Dis Rep. 2016 Jan 7;42(1):9-11. doi: 10.14745/ccdr.v42i01a02.
Enterovirus-D68 (EV-D68) was observed in association with severe respiratory disease in children in North America and around the world in the fall of 2014.
To compare fall 2014 detection rates with fall 2015 detection rates of EV-D68 in nasopharyngeal swab (NPS) samples collected for routine clinical care from a large regional laboratory in south-central Ontario.
Consecutive NPS samples submitted from inpatients and outpatients in Hamilton, Niagara Region and Burlington to the Regional Virology Laboratory were tested with multiplex polymerase chain reaction (PCR) for rhinovirus/enterovirus (as a single target) and for other common respiratory viruses. All NPS samples positive for rhinovirus/enterovirus were reflexed to a lab-developed single target PCR for EV-D68 detection.
In 2014, between August 1 and October 31, 566 of 1,497 (38%, 95%CI 35-40%) NPS samples were rhino/enterovirus positive, of which 177 (31%, 95%CI 27-35%) were confirmed as EV-D68. In 2015, between August 1 and October 31, 472 of 1,630 (29%, 95%CI 27-31%) NPS samples were rhino/enterovirus positive, of which none (0%, upper limit 97.5%CI 0.8%) were confirmed to be EV-D68.
Based on testing results, there appears to be much less circulating EV-D68 in south central Ontario in 2015 than in 2014. Further studies would be helpful to determine if detection rates have also dramatically decreased in other regions in Canada and internationally.
2014年秋季，在北美及世界各地均观察到肠道病毒D68型（EV-D68）与儿童严重呼吸道疾病有关。
比较2014年秋季与2015年秋季，从安大略省中南部一个大型区域实验室收集的用于常规临床护理的鼻咽拭子（NPS）样本中EV-D68的检出率。
将汉密尔顿、尼亚加拉地区和伯灵顿的住院患者和门诊患者提交至区域病毒学实验室的连续NPS样本，采用多重聚合酶链反应（PCR）检测鼻病毒/肠道病毒（作为单一靶点）以及其他常见呼吸道病毒。所有鼻病毒/肠道病毒呈阳性的NPS样本均采用实验室自行开发的单一靶点PCR进行EV-D68检测。
2014年8月1日至10月31日期间，1,497份NPS样本中有56,6份（38%，95%CI 35-40%）鼻病毒/肠道病毒呈阳性，其中177份（31%，95%CI 27-35%）被确认为EV-D68。2015年8月1日至10月31日期间，1,630份NPS样本中有472份（29%，95%CI 27-31%）鼻病毒/肠道病毒呈阳性，其中无一例（0%，上限97.5%CI 0.8%）被确认为EV-D68。
根据检测结果，2015年安大略省中南部地区循环传播的EV-D68似乎比2014年少得多。进一步的研究将有助于确定加拿大其他地区及国际上的检出率是否也大幅下降。
