Department of Food Science and Technology, University of California, Davis, USA.
Department of Nutrition, University of California, Davis, USA.
FEMS Microbiol Ecol. 2018 Jul 1;94(7). doi: 10.1093/femsec/fiy084.
We investigated whether sucrose metabolism by probiotic Lactobacillus plantarum influences the intestinal survival and microbial responses to this organism when administered to mice fed a sucrose-rich, Western diet. A L. plantarum mutant unable to metabolize sucrose was constructed by deleting scrB, coding for beta-fructofuranosidase, in a rifampicin-resistant strain of L. plantarum NCIMB8826. The ScrB deficient mutant survived in 8-fold higher numbers compared to the wild-type strain when measured 24 h after administration on two consecutive days. According to 16S rRNA marker gene sequencing, proportions of Faecalibacterium and Streptococcus were elevated in mice fed the L. plantarum ΔscrB mutant. Metagenome predictions also indicated those mice contained a higher abundance of lactate dehydrogenases. This was further supported by a trend in elevated fecal lactate concentrations among mice fed the ΔscrB mutant. L. plantarum also caused other changes to the fecal metabolomes including higher concentrations of glycerol in mice fed the ΔscrB mutant and increased uracil, acetate and propionate levels among mice fed the wild-type strain. Taken together, these results suggest that sucrose metabolism alters the properties of L. plantarum in the digestive tract and that probiotics can differentially influence intestinal metabolomes via their carbohydrate consumption capabilities.
我们研究了益生菌植物乳杆菌对蔗糖的代谢是否会影响其在喂食富含蔗糖的西式饮食的小鼠肠道中的存活和微生物反应。通过在植物乳杆菌 NCIMB8826 的 rifampicin 抗性菌株中缺失编码β-呋喃果糖苷酶的 scrB，构建了一种无法代谢蔗糖的植物乳杆菌突变体。与野生型菌株相比，该突变体在连续两天给药 24 小时后，存活数量增加了 8 倍。根据 16S rRNA 标记基因测序，喂食植物乳杆菌ΔscrB 突变体的小鼠中粪肠球菌和链球菌的比例升高。宏基因组预测还表明，这些小鼠含有更高丰度的乳酸脱氢酶。这一结果进一步得到了喂食ΔscrB 突变体的小鼠粪便中乳酸浓度升高的趋势的支持。植物乳杆菌还导致粪便代谢组发生其他变化，包括喂食ΔscrB 突变体的小鼠中甘油浓度升高，以及喂食野生型菌株的小鼠中尿嘧啶、乙酸和丙酸水平升高。综上所述，这些结果表明蔗糖代谢改变了肠道中植物乳杆菌的特性，并且益生菌可以通过其碳水化合物消耗能力差异地影响肠道代谢组。
