1 Department of Food Science and Technology, University of California, One Shields Avenue, Davis, CA 95616, USA.
2 Department of Plant Pathology, University of California, One Shields Avenue, Davis CA 95616-8751, USA.
Benef Microbes. 2018 Feb 27;9(2):333-344. doi: 10.3920/BM2017.0096. Epub 2017 Oct 25.
Plantaricin EF (PlnEF) is a class IIb bacteriocin produced by Lactobacillus plantarum. We compared L. plantarum NCIMB8826 and LM0419, a plnEFI deletion mutant of that strain lacking plnEF and the gene for the cognate immunity protein plnI, in a 2,4,6-trinitrobenzenesulfonic acid (TNBS) induced mouse model of acute inflammatory bowel disease. Mice fed either L. plantarum NCIMB8826 or LM0419 were not protected against TNBS according to either disease activity or histology (Ameho) scores. Mice consuming NCIMB8826 exhibited intermediate (non-significant) levels of colonic tumour necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) that ranged between the TNBS-treated animals and healthy controls. By comparison, TNF-α and IL-6 quantities were elevated in mice given L. plantarum LM0419 and equivalent to mice given TNBS alone. Both strains survived digestive tract transit in equal numbers and did not result in global changes to the bacterial composition in the intestine according to 16S rRNA gene sequencing either prior to or after TNBS administration. Examination of intestinal taxa showed that mice consuming wild-type L. plantarum, but not LM0419 contained lower proportions of Mucispirillum (Deferribacteres phylum) in the faeces prior to TNBS administration and Parabacteroides (Bacteroidetes phylum) in the caecum after disease induction. Parabacteroides also positively correlated with disease activity and histology scores. These findings suggest a role for PlnEFI production by L. plantarum in benefiting digestive tract health.
植物乳杆菌素 EF（PlnEF）是一种由植物乳杆菌产生的 IIb 类细菌素。我们比较了 L. plantarum NCIMB8826 和 LM0419，这是该菌株的 plnEFI 缺失突变体，缺乏 plnEF 和同源免疫蛋白 plnI 的基因，在 2,4,6-三硝基苯磺酸（TNBS）诱导的急性炎症性肠病小鼠模型中。无论是根据疾病活动度还是组织学（Ameho）评分，用 L. plantarum NCIMB8826 或 LM0419 喂养的小鼠都没有得到保护，免受 TNBS 的侵害。食用 NCIMB8826 的小鼠表现出中间（无统计学意义）水平的结肠肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6），介于 TNBS 处理的动物和健康对照之间。相比之下，给予 L. plantarum LM0419 的小鼠 TNF-α 和 IL-6 水平升高，与单独给予 TNBS 的小鼠相当。两种菌株在消化道转运中数量相等，并且根据 16S rRNA 基因测序，在给予 TNBS 之前或之后，都没有导致肠道内细菌组成发生全局变化。肠道分类群的检查表明，在给予 TNBS 之前，消耗野生型 L. plantarum 的小鼠但不是 LM0419 的粪便中含有较低比例的 Mucispirillum（厚壁菌门），而在疾病诱导后盲肠中含有较低比例的 Parabacteroides（拟杆菌门）。Parabacteroides 也与疾病活动度和组织学评分呈正相关。这些发现表明 L. plantarum 产生 PlnEF 对肠道健康有益。
