Zhu Feng, Yuan Chengshan, Gang Fangli, Yang Chaofu, Wu Wenjun, Zhang Jiwen
College of Chemistry & Pharmacy, Northwest A&F University, Yangling, 712100, P. R. China.
State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, P. R. China.
Chem Biodivers. 2018 Jul;15(7):e1800090. doi: 10.1002/cbdv.201800090. Epub 2018 Jun 21.
Oomycetes are one type of the most highly destructive of the diseases that cause damage to some important crop plants, such as potato late blight, cucumber downy mildew, and grape downy mildew. As main approach of the ongoing search for new botanical fungicide from plant, the secondary metabolites of D. aspersa were investigated. Through efficient bioassay-guided isolation, two new (1 and 2) and 12 known compounds (3 - 14) were isolated, and their structures were determined via extensive NMR, HR-ESI-MS, and IR. They were isolated from this genus for the first time except for compounds 11 and 12. The biological properties of 1 - 14 were evaluated against Pseudoperonospora cubensis and Phytophthora infestans. Compounds 1 - 8 showed potent antifungal activity in vitro. Additionally, compound 3 has preferable control effect on cucumber downy mildew, showing dual effect of protection and treatment in vivo.
卵菌纲是对一些重要农作物造成损害的最具破坏性的病害类型之一，如马铃薯晚疫病、黄瓜霜霉病和葡萄霜霉病。作为目前从植物中寻找新型植物源杀菌剂的主要途径，对黄斑盘菌的次生代谢产物进行了研究。通过高效的生物活性导向分离，分离得到了两个新化合物（1和2）和12个已知化合物（3 - 14），并通过广泛的核磁共振（NMR）、高分辨电喷雾电离质谱（HR-ESI-MS）和红外光谱（IR）确定了它们的结构。除化合物11和12外，它们首次从该属中分离得到。对化合物1 - 14针对古巴假霜霉和致病疫霉的生物学特性进行了评估。化合物1 - 8在体外显示出较强的抗真菌活性。此外，化合物3对黄瓜霜霉病具有较好的防治效果，在体内表现出保护和治疗的双重作用。
