Starling G C, Nimmo J C, Hart D N
Department of Haematology, Christchurch Hospital, New Zealand.
Pathology. 1988 Oct;20(4):361-5. doi: 10.3109/00313028809085220.
The hypothesis that interferon alpha (IFN alpha) has its beneficial effects in hairy cell leukemia by activating natural killer cells against hairy cells was examined. Leukemic cells from patients with hairy cell leukemia were tested for their susceptibility to lysis by fresh and IFN alpha activated peripheral blood mononuclear (PBMC) cells from normal donors. All hairy cells tested were relatively insensitive to cytolysis by PBMC and IFN alpha activated PBMC. The low levels of 51Cr release obtained with a few donors was due to lysis of leukemic cells, not residual normal cells, and was mediated by a natural killer cell (T cell receptor independent) mechanism. Chronic lymphatic leukemic cells before and after treatment with phorbol ester were also resistant to cytolysis. Hairy cells were not susceptible to lymphokine activated killer (LAK) cells but were sensitive to lysis by antibody and complement. The insensitivity to cell mediated cytolysis against hairy cells was shown by cold target inhibition to be a lack of target recognition by NK cells.
对干扰素α（IFNα）通过激活自然杀伤细胞对抗毛细胞从而在毛细胞白血病中发挥有益作用这一假说进行了研究。对毛细胞白血病患者的白血病细胞进行检测，观察其对来自正常供体的新鲜及经IFNα激活的外周血单个核（PBMC）细胞裂解的敏感性。所有检测的毛细胞对PBMC及经IFNα激活的PBMC的细胞溶解相对不敏感。少数供体获得的低水平51Cr释放是由于白血病细胞的裂解，而非残留的正常细胞，并且是由自然杀伤细胞（不依赖T细胞受体）机制介导的。经佛波酯处理前后的慢性淋巴细胞白血病细胞对细胞溶解也具有抗性。毛细胞对淋巴因子激活的杀伤（LAK）细胞不敏感，但对抗体和补体介导的裂解敏感。通过冷靶抑制表明，对毛细胞的细胞介导性细胞溶解不敏感是由于NK细胞缺乏靶识别。
