Laboratory of Cardiovascular Physiology and Reactive Oxygen Species, Physiology Department, Institute of Basic Health Sciences (ICBS), Federal University of Rio Grande do Sul (UFRGS), Brazil.
Biomed Pharmacother. 2018 Aug;104:165-171. doi: 10.1016/j.biopha.2018.05.031. Epub 2018 May 15.
The aim of this study was to analyse the effect of sulforaphane (SFN) in cultures of adult cardiomyocytes, evaluating oxidative stress at different times. Cells were isolated, cultured, and divided into 4 groups: Control, SFN (5μM), HO (5μM), and SFN+HO (5μM both), and subdivided into groups undergoing 1 or 24 h of SFN incubation. After 1 h of incubation, reactive oxygen species production was 40% lower in the SFN group than the Control, and lipid peroxidation was 63% higher in the HO group than the Control, and it was reduced in both of the SFN groups. The SOD activity was 59% higher in groups incubated for 24 h than in those incubated for 1 h. Protein expression of SOD-1 and SOD-2 was higher in the 24-h groups compared to the 1-h groups (55% and 24%, respectively). The Nrf2 protein expression in the 1-h groups was 17% higher than in the 24-h groups, and the SFN + HO group had 40% more Nrf2 than the Control in the 1-h groups. Unlike Nrf2, the PGC-1α expression was 69% higher in the 24-h groups in relation to the 1-h groups. Regarding the 24-h groups, the SFN and SFN+HO groups were higher than the Control (32% and 33%, respectively), and the SFN+HO group was increased (21%) compared to HO. SFN had a protective action against oxidative damage, but had no effect on the antioxidant enzymes analyzed. The different responses in the expression of Nrf2 and PGC-1α in relation to the incubation times, draws attention to the importance of establishing a timeline of the action of SFN, since there appears to be a temporal difference in its mechanism in adult cardiomyocytes.
本研究旨在分析萝卜硫素（SFN）对成年心肌细胞培养物的影响，评估不同时间的氧化应激。将细胞分离、培养并分为 4 组：对照组、SFN（5μM）组、HO（5μM）组和 SFN+HO（两者均为 5μM）组，并细分为进行 SFN 孵育 1 或 24 小时的组。孵育 1 小时后，SFN 组的活性氧产生比对照组低 40%，HO 组的脂质过氧化比对照组高 63%，而 SFN 组均有所降低。孵育 24 小时的组的 SOD 活性比孵育 1 小时的组高 59%。与 1 小时组相比，24 小时组的 SOD-1 和 SOD-2 蛋白表达分别高 55%和 24%。1 小时组的 Nrf2 蛋白表达比 24 小时组高 17%，而 SFN+HO 组在 1 小时组中的 Nrf2 比对照组高 40%。与 Nrf2 不同，PGC-1α 在 24 小时组中的表达比 1 小时组高 69%。对于 24 小时组，SFN 和 SFN+HO 组均高于对照组（分别为 32%和 33%），SFN+HO 组与 HO 组相比也有所增加（21%）。SFN 对氧化损伤具有保护作用，但对分析的抗氧化酶没有影响。Nrf2 和 PGC-1α 的表达在孵育时间方面的不同反应引起了对 SFN 作用的时间进程的重视，因为在成年心肌细胞中，它的作用机制似乎存在时间差异。
