Campisciano Giuseppina, Zanotta Nunzia, Petix Vincenzo, Corich Lucia, De Seta Francesco, Comar Manola
Institute for Maternal and Child Health IRCCS, Burlo Garofolo, Via dell' Istria 65/1, Trieste, 34137 Italy.
Institute for Maternal and Child Health - IRCCS Burlo Garofolo, Via dell'Istria 65/1, Trieste, 34137 Italy.
Front Biosci (Elite Ed). 2018 Jun 1;10(3):528-536. doi: 10.2741/E839.
Bacterial vaginosis involves the presence of a polymicrobial biofilm on the vaginal epithelium, guaranteeing immune escape and spread of antibiotic resistance. To spot known biofilm-forming bacteria, we profiled the vaginal microbiome of sixty-four symptomatic women suffering from a different grade of vaginal disorders and sixty asymptomatic healthy women. Specific microbial profiles distinguished symptomatic from asymptomatic women and characterized the grade of dysmicrobism within the symptomatic group. Lactobacillus crispatus and iners predominated on the healthy vaginal mucosa, while Lactobacillus gasseri predominated in the intermediate dysmicrobism. Furthermore, the intermediate grade of dysmicrobism was characterized by other lactic acid-producers species than Lactobacilli, able to rescue the microbial imbalance, and Ureaplasma parvum-serovar 3. The vaginosis group exhibited the overgrowth of Prevotella bivia, which is known to enhance the biofilm formation by Gardnerella vaginalis, and the presence of Streptococcus anginosus, which is emerging as a new cooperating player of the vaginal biofilm. Identifying specific microorganisms promoting or preventing the biofilm formation could increase the accuracy for a better definition of the vaginal dysmicrobism concept and therapeutic intervention.
细菌性阴道病涉及阴道上皮上存在多微生物生物膜，这确保了免疫逃逸和抗生素耐药性的传播。为了找出已知的形成生物膜的细菌，我们对64名患有不同程度阴道疾病的有症状女性和60名无症状健康女性的阴道微生物群进行了分析。特定的微生物谱区分了有症状和无症状女性，并确定了有症状组内微生物失调的程度。卷曲乳杆菌和惰性乳杆菌在健康阴道黏膜中占主导地位，而加氏乳杆菌在中度微生物失调中占主导地位。此外，中度微生物失调的特征还包括除乳杆菌外的其他产乳酸菌种，它们能够纠正微生物失衡，以及微小脲原体血清型3。阴道病组表现出二路普雷沃菌过度生长，已知该菌可增强阴道加德纳菌的生物膜形成，还存在咽峡炎链球菌，它正成为阴道生物膜的新协同参与者。识别促进或阻止生物膜形成的特定微生物，可能会提高对阴道微生物失调概念和治疗干预进行更好定义的准确性。
