Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China.
National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2018 May 16;19(5):1481. doi: 10.3390/ijms19051481.
Abscisic acid (ABA) plays an important role in the regulation of plant adaptation, seed germination, and root development in plants. However, the mechanism of ABA regulation of root development is still poorly understood, especially through the miRNA-mediated pathway Here, small RNA (sRNA)-seq and degradome-seq were used to analyze the miRNAs' responsive to ABA in the stems and roots of , a model tree species for abiotic stress-resistance research. In total, 255 unique mature sequences, containing 154 known miRNAs and 101 novel miRNAs were identified, among which 33 miRNAs and 54 miRNAs were responsive to ABA in the roots and stems, respectively. Furthermore, the analysis of these miRNAs and their targets revealed a new hormone signaling crosstalk model of ABA regulation of root growth through miRNA-mediated pathways, such as peu-miR-n68 mediation of the crosstalk between ABA and the brassinosteroid (BR) signaling pathway and peu-miR477b mediation of the crosstalk between ABA and Gibberellic acid (GA) signaling. Taken together, our genome-wide analysis of the miRNAs provides a new insight into the mechanism of ABA regulation of root growth in .
脱落酸（ABA）在植物适应、种子萌发和根系发育的调节中起着重要作用。然而，ABA 调节根系发育的机制仍知之甚少，特别是通过 miRNA 介导的途径。在这里，使用小 RNA（sRNA）-seq 和降解组-seq 分析了模式树种对非生物胁迫研究具有抗逆性的 中 ABA 响应的茎和根中的 miRNA。总共鉴定出 255 个独特的成熟序列，包含 154 个已知的 miRNA 和 101 个新的 miRNA，其中 33 个 miRNA 和 54 个 miRNA 分别在根和茎中对 ABA 有响应。此外，这些 miRNA 及其靶标的分析揭示了 ABA 通过 miRNA 介导的途径调节根生长的新激素信号转导串扰模型，如 peu-miR-n68 介导 ABA 与油菜素内酯（BR）信号通路的串扰和 peu-miR477b 介导 ABA 与赤霉素（GA）信号通路的串扰。总之，我们对 miRNA 的全基因组分析为 ABA 调节 的根生长机制提供了新的见解。
